Characterisation of the symbionts in the Mediterranean fruit fly gut.
Characterisation of the symbionts in the Mediterranean fruit fly gut.
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DOI：
10.1099/mgen.0.000801
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发表时间：
2022-04
影响因子：
3.9
通讯作者：
Chapman, Tracey
中科院分区：
文献类型：
--
作者：
Darrington, Mike;Leftwich, Philip T.;Holmes, Neil A.;Friend, Lucy A.;Clarke, Naomi V. E.;Worsley, Sarah F.;Margaritopolous, John T.;Hogenhout, Saskia A.;Hutchings, Matthew I.;Chapman, Tracey
细菌和它们的昆虫宿主之间的共生可以从松散的关联到专有的相互依赖。虽然对专性互惠关系的深入研究已经获得了基本的进化见解，但人们对宿主与其肠道微生物群之间灵活的共生关系的进化潜力越来越感兴趣。了解微生物和宿主之间的关系也提供了利用昆虫控制的潜力。在这里，我们调查了一种全球农业害虫--地中海果蝇(Certis Capata)的肠道微生物群。我们使用16S rRNA图谱比较了不同饲料饲养的实验室菌株和野生菌株以及从不同自然植物寄主采集的苍蝇的肠道微生物群。结果表明，蚂蚁的肠道中有一个简单的微生物群，这主要由幼虫的饮食决定。然而，无论是实验室的食物还是饲养苍蝇的天然植物宿主，克雷伯氏菌都是如此。占主导地位的麦德蝇微生物群，并对抗生素治疗清除具有抵抗力。我们对优势克雷伯氏菌的基因组进行了测序。(‘Medkleb’)从Toliman野生型菌株的肠道中分离出来。全基因组ANI分析将Medkleb归入催产克雷伯菌/密歇根克雷伯氏菌组。Medkleb的物种水平分类是用多位点系统发育方法解决的--分子、序列和表型分析都支持它是K.Michiganensis。Medkleb的基因组大小(5825435 碱基)比自由生活的克雷伯氏菌的平均基因组大小小1.6个标准差。Medkleb也缺乏一些与环境感知有关的基因。此外，Medkleb基因组包含至少两个最近获得的独特基因组岛，以及编码能够降解植物细胞壁的果胶分解酶的基因。这可能是有利的，因为苍蝇的饮食包括含有高比例果胶的未成熟水果。结果表明，蚂蚁体内有一种共生肠道细菌，这种细菌可能已经与宿主建立了互惠互利的关系，并提供了营养益处。
Symbioses between bacteria and their insect hosts can range from loose associations through to obligate interdependence. While fundamental evolutionary insights have been gained from the in-depth study of obligate mutualisms, there is increasing interest in the evolutionary potential of flexible symbiotic associations between hosts and their gut microbiomes. Understanding relationships between microbes and hosts also offers the potential for exploitation for insect control. Here, we investigate the gut microbiome of a global agricultural pest, the Mediterranean fruit fly (Ceratitis capitata). We used 16S rRNA profiling to compare the gut microbiomes of laboratory and wild strains raised on different diets and from flies collected from various natural plant hosts. The results showed that medfly guts harbour a simple microbiome that is primarily determined by the larval diet. However, regardless of the laboratory diet or natural plant host on which flies were raised, Klebsiella spp. dominated medfly microbiomes and were resistant to removal by antibiotic treatment. We sequenced the genome of the dominant putative Klebsiella spp. (‘Medkleb’) isolated from the gut of the Toliman wild-type strain. Genome-wide ANI analysis placed Medkleb within the K. oxytoca / michiganensis group. Species level taxonomy for Medkleb was resolved using a mutli-locus phylogenetic approach - and molecular, sequence and phenotypic analyses all supported its identity as K. michiganensis . Medkleb has a genome size (5825435 bp) which is 1.6 standard deviations smaller than the mean genome size of free-living Klebsiella spp. Medkleb also lacks some genes involved in environmental sensing. Moreover, the Medkleb genome contains at least two recently acquired unique genomic islands as well as genes that encode pectinolytic enzymes capable of degrading plant cell walls. This may be advantageous given that the medfly diet includes unripe fruits containing high proportions of pectin. The results suggest that the medfly harbours a commensal gut bacterium that may have developed a mutualistic association with its host and provide nutritional benefits.
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影响因子：
3.2
作者：
Craig, Jeffrey W.;Cherry, Marisa A.;Brady, Sean F.
通讯作者：
Brady, Sean F.
影响因子：
4.2
作者：
de Souza DJ;Bézier A;Depoix D;Drezen JM;Lenoir A
通讯作者：
Lenoir A
影响因子：
3.3
作者：
Bennett GM;Moran NA
通讯作者：
Moran NA
影响因子：
4.9
作者：
Behar, A.;Jurkevitch, E.;Yuval, B.
通讯作者：
Yuval, B.
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob