Small, smaller, smallest: the origins and evolution of ancient dual symbioses in a Phloem-feeding insect.
Small, smaller, smallest: the origins and evolution of ancient dual symbioses in a Phloem-feeding insect.
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DOI：
10.1093/gbe/evt118
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发表时间：
2013
影响因子：
3.3
通讯作者：
Moran NA
中科院分区：
文献类型：
--
作者：
Bennett GM;Moran NA
许多昆虫依赖于细菌共生体，这些细菌共生体具有专门用于提供宿主饮食中缺乏的营养的微小基因组。木质部汁液和韧皮部汁液作为昆虫的食物都是缺乏的，但是营养成分显著不同，潜在地影响共生体基因组的进化。对于取食树液的昆虫，共生体基因组序列仅可用于胸喙亚目的韧皮部取食实例和Auchellehyncha亚目的木质部取食实例，混淆比较。我们测序的专性共生体，Sulcia muelleri和Nasuia deltocephalinicola，的韧皮部喂养害虫，Macrosteles quadrilineatus（Auchelirhyncha：Cicadellidae）的基因组。我们的研究结果表明，Nasuia-ALF具有最小的细菌基因组测序（112 kb），Sulcia-ALF基因组（190 kb）小于Sulcia在其他昆虫谱系。总之，这些共生体保留了合成10种必需氨基酸的能力，正如从以木质部为食的Auchalphrhyncha中观察到的几种共生体对一样。Nasuia保留了能够合成两种氨基酸、DNA复制、转录和翻译的基因。这两种共生体都失去了通过氧化磷酸化合成ATP的基因，这可能是韧皮部富含糖的结果。共有的基因组特征，包括UGA密码子从终止到色氨酸的重新分配，以及系统发育结果表明，Nasuia-ALF与Zinderia最密切相关，Zinderia是spittlebugs的betaproteobacterial共生体。因此，Nasuia/Zinderia和Sulcia可能代表了自叶蝉和沫蝉分化以来（>200 Ma），以及可能自Aucheliphrhyncha起源以来（>260 Ma），共同居住在宿主中的古老伙伴。
Many insects rely on bacterial symbionts with tiny genomes specialized for provisioning nutrients lacking in host diets. Xylem sap and phloem sap are both deficient as insect diets, but differ dramatically in nutrient content, potentially affecting symbiont genome evolution. For sap-feeding insects, sequenced symbiont genomes are available only for phloem-feeding examples from the suborder Sternorrhyncha and xylem-feeding examples from the suborder Auchenorrhyncha, confounding comparisons. We sequenced genomes of the obligate symbionts, Sulcia muelleri and Nasuia deltocephalinicola, of the phloem-feeding pest insect, Macrosteles quadrilineatus (Auchenorrhyncha: Cicadellidae). Our results reveal that Nasuia-ALF has the smallest bacterial genome yet sequenced (112 kb), and that the Sulcia-ALF genome (190 kb) is smaller than that of Sulcia in other insect lineages. Together, these symbionts retain the capability to synthesize the 10 essential amino acids, as observed for several symbiont pairs from xylem-feeding Auchenorrhyncha. Nasuia retains genes enabling synthesis of two amino acids, DNA replication, transcription, and translation. Both symbionts have lost genes underlying ATP synthesis through oxidative phosphorylation, possibly as a consequence of the enriched sugar content of phloem. Shared genomic features, including reassignment of the UGA codon from Stop to tryptophan, and phylogenetic results suggest that Nasuia-ALF is most closely related to Zinderia, the betaproteobacterial symbiont of spittlebugs. Thus, Nasuia/Zinderia and Sulcia likely represent ancient associates that have co-resided in hosts since the divergence of leafhoppers and spittlebugs >200 Ma, and possibly since the origin of the Auchenorrhyncha, >260 Ma.
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影响因子：
3.3
作者：
Alves JM;Serrano MG;Maia da Silva F;Voegtly LJ;Matveyev AV;Teixeira MM;Camargo EP;Buck GA
通讯作者：
Buck GA
影响因子：
2.2
作者：
BRODBECK, BV;MIZELL, RF;ANDERSEN, PC
通讯作者：
ANDERSEN, PC
影响因子：
23.8
作者：
Arrese EL;Soulages JL
通讯作者：
Soulages JL
DOI：
10.1073/pnas.1533499100
发表时间：
2003-08-05
影响因子：
11.1
作者：
Gil, R;Silva, FJ;Moya, A
通讯作者：
Moya, A
影响因子：
2.2
作者：
Frost, K. E.;Willis, D. K.;Groves, R. L.
通讯作者：
Groves, R. L.