Graphene Oxide-Based Biocompatible 3D Mesh with a Tunable Porosity and Tensility for Cell Culture.
Graphene Oxide-Based Biocompatible 3D Mesh with a Tunable Porosity and Tensility for Cell Culture.
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DOI：
10.1021/acsbiomaterials.8b00190
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发表时间：
2018-05-14
影响因子：
5.8
通讯作者：
Zhao JX
中科院分区：
文献类型：
--
作者：
Zhang Y;Liu X;Michelson K;Trivedi R;Wu X;Schepp E;Xing Y;Darland D;Zhao JX
One of the major challenges associated with modeling the influence of the cellular microenvironment on cell growth and differentiation is finding suitable substrates for growing the cells in a manner that recapitulates the cell–cell and cell–microenvironmental interactions in vitro. As one approach to address this challenge, we have developed graphene oxide (GO)-3D mesh with tunable hardness and porosity for application in cell culture systems. The synthetic method of GO-3D mesh is simple, easily reproducible, and low cost. The foundation of the method is the combination of poly(ethylene)(glycol) (PEG) and GO together with a salt-leaching approach (NaCl) in addition to a controlled application of heat during the synthetic process to tailor the mechanical properties, porosity, and pore-size distribution of the resulting GO-3D mesh. With this methodology, the hydrogel formed by PEG and GO generates a microporous mesh in the presence of the NaCl, leading to the formation of a stable 3D scaffold after extensive heating and washing. Varying the ratio of NaCl to GO controls porosity, pore size, and pore connectivity for the GO-3D mesh. When the porosity is less than 90%, with an increasing ratio of NaCl to GO, the number of pores increases with good interconnectivity. The 3D-mesh showed excellent biocompatibility with vascular cells which can take on a morphology comparable to that observed in vessels in vivo. Cell proliferation and gene expression can be determined from cells grown on the GO-3D scaffold, providing a valuable tool for investigating cell–microenvironmental changes. The GO-3D mesh described results from the synergy of the combined chemical properties of the PEG and GO with the salt-leaching methodology to generate a unique and flexible mesh that can be modified and optimized for a variety of in vitro applications.
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影响因子：
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作者：
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