Differential expression patterns of N-acetylglucosaminyl transferases and polylactosamines in uterine lesions.
Differential expression patterns of N-acetylglucosaminyl transferases and polylactosamines in uterine lesions.
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DOI：
10.4081/ejh.2014.2334
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发表时间：
2014-05-26
期刊：
影响因子：
--
通讯作者：
Beltrão EI
中科院分区：
文献类型：
--
作者：
Clark AT;Guimarães da Costa VM;Bandeira Costa L;Bezerra Cavalcanti CL;De Melo Rêgo MJ;Beltrão EI
聚乳糖胺(PolyLacNAc)是糖结合物中的一种基本结构，在特定的细胞或组织中表达，与肿瘤的发生和发展有关。β1，3-N-乙酰氨基葡萄糖转移酶(β3Gnts)在聚乳酸合成中起重要作用，但这些糖基转移酶及其产物在肿瘤进展中的作用尚不清楚。在这个意义上，这项工作旨在评估N-乙酰氨基葡萄糖转移酶和聚乳糖胺在宫颈浸润性病变和癌前病变中的差异表达模式。采用免疫组织化学方法检测β3GnT2和β3GnT3在正常宫颈(n=10)、宫颈病变(n=12 0)、宫颈鳞癌(SC)和癌前病变[宫颈上皮内瘤变(CIN)，1、2、3级]中的表达。此外，还用商陆凝集素(PWM)和小麦胚芽凝集素(WGA)进行了凝集素组织化学，分别观察了多聚LacNAc链和N-乙酰氨基葡萄糖(GlcNAc)的存在。β3GnT3几乎全部表达(99%)，β3GnT2主要在CIN 3中表达，高于正常(P=0.002)、CIN 1(P=0.009)和CIN 2(P=0.03)。PolyLacNAc在鳞癌组织中的表达高于正常组(P=0.03)、CIN 1组(P=0.02)和CIN 3组(P=0.004)，但仅有50%的SC组有核表达，与正常组(P=0.01)、CIN 1组(P=0.002)、CIN 2组(P=0.007)和CIN 3组(P=0.04)相比，差异有统计学意义。与转移酶和多聚LacNAc链不同，GlcNAc(WGA配体)随着病变程度的增加而逐渐减少，在CIN 1皮损中的表达高于正常(P<；0.0001)、CIN 2(P<；0.0001)、SC(P<；0.0001)和CIN 3(P=0.0003)。我们的数据显示β3GnT2和PolyLacNAc可能参与了人类宫颈癌前病变的进展。此外，在子宫病变中，PolyLacNAc仅在细胞核中表达可能与预后不良相关。
Polylactosamine (polyLacNAc) is a fundamental structure in glycoconjugates and it is expressed in specific cells/tissues associated with the development and carcinogenesis. β1,3-N-acetylglucosaminyl transferases ((β3GnTs) play an important role in polyLacNAc synthesis, however the roles of these glycosyltransferases and their products in cancer progression are still unclear. In this sense, this work aimed to evaluate differential expression pattern of the N-acetylglucosaminyl transferases and polylactosamines in invasive and premalignant lesions of the uterus cervix. The expression of β3GnT2 and β3GnT3 were evaluated in normal (n=10) and uterine cervix lesions (n=120), both malignant [squamous carcinoma (SC)] and premalignant [cervical intraepithelial neoplasia (CIN), grades 1, 2 and 3] using immunohistochemistry. Besides, lectin histochemistry with Phytolacca americana lectin (PWM) and Wheat germ agglutinin (WGA) was also carried out to observe the presence of polyLacNAc chains and N-acetylglucosamine (GlcNAc), respectively. The β3GnT3 was expressed in almost all samples (99%) and β3GnT2 was higher expressed in disease samples mainly in CIN 3, when compared with normal (P=0.002), CIN 1 (P=0.009) and CIN 2 (P=0.03). The expression of polyLacNAc was higher is SC samples, when compared with normal (P=0.03), CIN 1 (P=0.02) and CIN 3 (P=0.004), and was observed only nuclear expression in nearly 50% of the SC samples, showing a statistically significant when compared with normal (P=0.01), CIN 1 (P=0.002), CIN 2 (P=0.007) and CIN 3 (P=0.04). Deferring from transferases and polyLacNAc chains, GlcNAc (WGA ligand) reveals a gradual staining pattern decrease with the increase of the lesion degree, being more expressed in CIN 1 lesions when compared with normal (P<0.0001), CIN 2 (P<0.0001), SC (P<0.0001) and CIN 3 (P=0.0003). Our data reveal that β3GnT2 and polyLacNAc may be involved in the progression of the pre-malignant lesions of the human uterine cervix. In addition, polyLacNAc expression only in the nucleus can be associated a poor prognostic in uterine lesions.
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影响因子：
4.1
作者：
Dojima, Takashi;Nishina, Takuya;Park, Enoch Y.
通讯作者：
Park, Enoch Y.
DOI：
10.1016/j.bbrc.2005.02.033
发表时间：
2005-04-08
影响因子：
3.1
作者：
Kato, T;Suzuki, M;Park, EY
通讯作者：
Park, EY
DOI：
10.1590/s1676-24442005000600009
发表时间：
2005-12-01
影响因子：
--
作者：
Lopes, Fernanda Ferreira;Cruz, Maria Carmen Fontoura Nogueira de;Freitas, Roseana de Almeida
通讯作者：
Freitas, Roseana de Almeida
DOI：
10.1186/bcr75
发表时间：
2000
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Couldrey C;Green JE
通讯作者：
Green JE
影响因子：
3.5
作者：
Ishida, H;Togayachi, A;Narimatsu, H
通讯作者：
Narimatsu, H