Msx1(+) stem cells recruited by bioactive tissue engineering graft for bone regeneration.
Msx1(+) stem cells recruited by bioactive tissue engineering graft for bone regeneration.
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DOI：
10.1038/s41467-022-32868-y
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发表时间：
2022-09-05
影响因子：
16.6
通讯作者：
Ouyang, Hongwei
中科院分区：
文献类型：
--
作者：
Zhang, Xianzhu;Jiang, Wei;Xie, Chang;Wu, Xinyu;Ren, Qian;Wang, Fei;Shen, Xilin;Hong, Yi;Wu, Hongwei;Liao, Youguo;Zhang, Yi;Liang, Renjie;Sun, Wei;Gu, Yuqing;Zhang, Tao;Chen, Yishan;Wei, Wei;Zhang, Shufang;Zou, Weiguo;Ouyang, Hongwei
临界尺寸的骨缺损通常导致骨不连，颅骨的全层缺损特别是仍然存在重建挑战。在这项研究中，我们发现神经营养补充剂诱导间充质基质细胞的体外扩增，并且神经营养补充剂结合3D打印水凝胶移植物的原位移植促进了临界尺寸骨缺损的全层再生。单细胞RNA测序分析表明，在体内颅骨愈合过程中产生了独特的原位干/祖细胞图谱。值得注意的是，我们发现本地扩展居民Msx 1+骨骼干细胞移植后的原位细胞培养系统。此外，增强的颅骨骨再生伴随着增加的软骨内骨化，这与Msx 1+骨骼干细胞密切相关。我们的研究结果说明了针对体内主要细胞亚群的原位细胞培养系统用于快速骨组织再生的省时和再生功效。临界尺寸的骨缺损仍然存在临床挑战。在这里，作者表明，移植神经营养因子掺入的水凝胶移植物促进了颅骨的全层再生，并进行scRNA-seq以揭示贡献的干/祖细胞，特别是常驻Msx 1+骨骼干细胞群。
Critical-sized bone defects often lead to non-union and full-thickness defects of the calvarium specifically still present reconstructive challenges. In this study, we show that neurotrophic supplements induce robust in vitro expansion of mesenchymal stromal cells, and in situ transplantation of neurotrophic supplements-incorporated 3D-printed hydrogel grafts promote full-thickness regeneration of critical-sized bone defects. Single-cell RNA sequencing analysis reveals that a unique atlas of in situ stem/progenitor cells is generated during the calvarial bone healing in vivo. Notably, we find a local expansion of resident Msx1+ skeletal stem cells after transplantation of the in situ cell culture system. Moreover, the enhanced calvarial bone regeneration is accompanied by an increased endochondral ossification that closely correlates to the Msx1+ skeletal stem cells. Our findings illustrate the time-saving and regenerative efficacy of in situ cell culture systems targeting major cell subpopulations in vivo for rapid bone tissue regeneration. Critical-sized bone defects still present clinical challenges. Here the authors show that transplantation of neurotrophic supplement-incorporated hydrogel grafts promote full-thickness regeneration of the calvarium and perform scRNA-seq to reveal contributing stem/progenitor cells, notably a resident Msx1+ skeletal stem cell population.
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