An Aptamer-Based Proteomic Analysis of Plasma from Cats (Felis catus) with Clinical Feline Infectious Peritonitis.
An Aptamer-Based Proteomic Analysis of Plasma from Cats (Felis catus) with Clinical Feline Infectious Peritonitis.
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临床猫传染性腹膜炎猫（猫卡图斯）血浆的基于适体的蛋白质组学分析。
DOI：
10.3390/v16010141
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发表时间：
2024-01-18
期刊：
影响因子：
--
通讯作者：
Dean GA
中科院分区：
文献类型：
--
作者：
Curtis BE;Abdo Z;Graham B;LaVoy A;Evans SJM;Santangelo K;Dean GA
猫传染性腹膜炎（FIP）是猫冠状病毒（FCoV）的一种全身性疾病表现，是家猫中与传染病相关死亡的最重要原因。FIP具有多样的临床表现，但最常见的特征是广泛的血管炎伴内脏受累和/或神经系统疾病，在没有抗病毒治疗的情况下通常是致命的。我们使用基于适体的蛋白质组学分析方法，分析了自然感染FIP的猫（n = 19）的血浆蛋白谱，并与临床健康且FCoV阴性的猫（n = 17）以及肠道型FCoV阳性的猫（n = 9）的血浆蛋白谱进行了比较。我们鉴定出442种有显著差异的蛋白质；与临床健康猫的血浆相比，FIP血浆中共有219种蛋白质增加，223种蛋白质减少。通路富集及相关分析表明，有差异的蛋白质与免疫系统过程有关，包括先天免疫应答、细胞因子信号传导和抗原呈递，以及细胞凋亡和血管完整性。我们在以往研究的背景下讨论了这些发现的相关性。虽然这些结果有可能为诊断、治疗和预防研究提供信息，但它们只是第一步，还需要进一步验证。
Feline infectious peritonitis (FIP) is a systemic disease manifestation of feline coronavirus (FCoV) and is the most important cause of infectious disease-related deaths in domestic cats. FIP has a variable clinical manifestation but is most often characterized by widespread vasculitis with visceral involvement and/or neurological disease that is typically fatal in the absence of antiviral therapy. Using an aptamer-based proteomics assay, we analyzed the plasma protein profiles of cats who were naturally infected with FIP (n = 19) in comparison to the plasma protein profiles of cats who were clinically healthy and negative for FCoV (n = 17) and cats who were positive for the enteric form of FCoV (n = 9). We identified 442 proteins that were significantly differentiable; in total, 219 increased and 223 decreased in FIP plasma versus clinically healthy cat plasma. Pathway enrichment and associated analyses showed that differentiable proteins were related to immune system processes, including the innate immune response, cytokine signaling, and antigen presentation, as well as apoptosis and vascular integrity. The relevance of these findings is discussed in the context of previous studies. While these results have the potential to inform diagnostic, therapeutic, and preventative investigations, they represent only a first step, and will require further validation.
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影响因子：
3.3
作者：
Vermeulen BL;Devriendt B;Olyslaegers DA;Dedeurwaerder A;Desmarets LM;Favoreel HW;Dewerchin HL;Nauwynck HJ
通讯作者：
Nauwynck HJ
影响因子：
3.3
作者：
Dean GA;Olivry T;Stanton C;Pedersen NC
通讯作者：
Pedersen NC
影响因子：
14.9
作者：
Gillespie M;Jassal B;Stephan R;Milacic M;Rothfels K;Senff-Ribeiro A;Griss J;Sevilla C;Matthews L;Gong C;Deng C;Varusai T;Ragueneau E;Haider Y;May B;Shamovsky V;Weiser J;Brunson T;Sanati N;Beckman L;Shao X;Fabregat A;Sidiropoulos K;Murillo J;Viteri G;Cook J;Shorser S;Bader G;Demir E;Sander C;Haw R;Wu G;Stein L;Hermjakob H;D'Eustachio P
通讯作者：
D'Eustachio P
影响因子：
2.7
作者：
Benjamini, Yoav;Krieger, Abba M.;Yekutieli, Daniel
通讯作者：
Yekutieli, Daniel
影响因子：
4.4
作者：
Watanabe R;Eckstrand C;Liu H;Pedersen NC
通讯作者：
Pedersen NC