Characterization of peritoneal cells from cats with experimentally-induced feline infectious peritonitis (FIP) using RNA-seq.
Characterization of peritoneal cells from cats with experimentally-induced feline infectious peritonitis (FIP) using RNA-seq.
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DOI：
10.1186/s13567-018-0578-y
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发表时间：
2018-08-07
影响因子：
4.4
通讯作者：
Pedersen NC
中科院分区：
文献类型：
--
作者：
Watanabe R;Eckstrand C;Liu H;Pedersen NC
实验室猫被FIP病毒（FIPV）的血清型I猫的场菌株感染，并在淋巴细胞症发作，发烧和血清炎时通过腹膜腔收集了2-3周的腹膜细胞。巨噬细胞在两个种群中占主导地位。与正常的猫相比，显示的956个基因> 2.0 log2折叠（log2fc）和1589个基因<−2.0 log2fc。 -STAT信号传导，NK细胞介导的细胞毒性，几种慢性传染病，移植物与宿主基于RNA表达的模式，疾病，同类排斥和某些自身免疫性疾病被激活的M1类型。 。在其他冠状病毒中鉴定出来的（APN，L-签名），而不是放松调节（DDP-4）或下调（DC-SIGN），但是，FCγRIIIA的mRNA（CD16A/ADCC受体）被显着更新，并被显着更新（支持病毒作为一种免疫复合物。以前与FIP巨噬细胞相关的基因往往会改变这种感知。
Laboratory cats were infected with a serotype I cat-passaged field strain of FIP virus (FIPV) and peritoneal cells harvested 2–3 weeks later at onset of lymphopenia, fever and serositis. Comparison peritoneal cells were collected from four healthy laboratory cats by peritoneal lavage and macrophages predominated in both populations. Differential mRNA expression analysis identified 5621 genes as deregulated in peritoneal cells from FIPV infected versus normal cats; 956 genes showed > 2.0 Log2 Fold Change (Log2FC) and 1589 genes showed < −2.0 Log2FC. Eighteen significantly upregulated pathways were identified by InnateDB enrichment analysis. These pathways involved apoptosis, cytokine–cytokine receptor interaction, pathogen recognition, Jak-STAT signaling, NK cell mediated cytotoxicity, several chronic infectious diseases, graft versus host disease, allograft rejection and certain autoimmune disorders. Infected peritoneal macrophages were activated M1 type based on pattern of RNA expression. Apoptosis was found to involve large virus-laden peritoneal macrophages more than less mature macrophages, suggesting that macrophage death played a role in virus dissemination. Gene transcripts for MHC I but not II receptors were upregulated, while mRNA for receptors commonly associated with virus attachment and identified in other coronaviruses were either not detected (APN, L-SIGN), not deregulated (DDP-4) or down-regulated (DC-SIGN). However, the mRNA for FcγRIIIA (CD16A/ADCC receptor) was significantly upregulated, supporting entry of virus as an immune complex. Analysis of KEGG associated gene transcripts indicated that Th1 polarization overshadowed Th2 polarization, but the addition of relevant B cell associated genes previously linked to FIP macrophages tended to alter this perception.
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影响因子：
3.3
作者：
Dean GA;Olivry T;Stanton C;Pedersen NC
通讯作者：
Pedersen NC
影响因子：
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作者：
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通讯作者：
Iwakura, Y