The Elevated Expressions of Anti-lipopolysaccharide Factors After Priming Stimulation Confer Lastingly Humoral Protection in Crab Eriocheir sinensis.
The Elevated Expressions of Anti-lipopolysaccharide Factors After Priming Stimulation Confer Lastingly Humoral Protection in Crab Eriocheir sinensis.
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启动刺激后抗脂多糖因子表达升高，为中华绒螯蟹提供持久的体液保护
DOI：
10.3389/fimmu.2021.757434
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发表时间：
2021
影响因子：
7.3
通讯作者：
Song L
中科院分区：
文献类型：
--
作者：
Wang W;Li Y;Fan S;Lian X;Cao W;Song X;Yi Q;Wang L;Song L
无脊椎动物的免疫记忆（免疫启动）的证据已经在各种生物中积累，细胞和体液免疫反应被推测参与免疫启动。然而，对所涉及的分子机制缺乏了解。在本研究中，预处理血淋巴的保护作用进一步验证了接受预处理血淋巴输注的幼稚蟹的存活率增加。通过蛋白质组学分析，从致敏血淋巴中鉴定出474个蛋白质，其中大部分在运输和代谢类中进行了功能注释。结果表明，在嗜水气单胞菌致敏后12 h和7 d，血淋巴中共有70个蛋白质表达差异，其中抗脂多糖因子1（EsALF-1）和抗脂多糖因子3（EsALF-3）表达差异最显著（p < 0.05）。在与A.与空白蟹相比，Escherichia、EsALF-1和EsALF-3在mRNA（血细胞）和蛋白质（血淋巴）水平均高表达，并且EsTLR 1-EsMyd 88-EsPelle-EsALF通路中各组分的mRNA表达量也显著增加（p <0. 05）。EsALF-3和EsMyd 88在第二次A. RNAi敲除EsTLR 1后，它们的表达均下降（p < 0.05）。注射重组蛋白EsALF-1（rEsALF-1）或EsALF-3（rEsALF-3）后，幼蟹的存活率显著提高（p < 0.05）。嗜水气刺激。相反，在接受EsALF-1或EsALF-3的抗体注射后，致敏蟹的存活率显著降低（p < 0.05）。A.预激刺激可持续4周。以上结果提示，血淋巴中EsTLR 1介导的EsALF-1和EsALF-3的产生在A.这为进一步了解无脊椎动物的免疫记忆提供了可靠的证据。
Evidence of immune memory in invertebrates (immune priming) has accumulated in various organisms, and both cellular and humoral immune reactions are speculated to be involved in immune priming. However, there is a lack of understanding of the molecular mechanisms involved. In the present study, the protective effect of primed haemolymph was further validated by the increased survival rate of naïve crabs receiving a transfusion of primed haemolymph. By proteomic analysis, there were 474 proteins identified from the primed haemolymph, and most of them were functionally annotated in transport and metabolism classes. A total of 70 proteins were found to be differentially expressed in haemolymph at 12 hours and 7 days after priming stimulation with Aeromonas hydrophila, among which anti-lipopolysaccharide factor 1 (EsALF-1) and 3 (EsALF-3) were identified as the most significant (p < 0.05). After being challenged with A. hydrophila, EsALF-1 and EsALF-3 were highly expressed at both mRNA (in haemocytes) and protein (in haemolymph) levels compared with blank crabs, and the mRNA expressions of components in the EsTLR1-EsMyd88-EsPelle-EsALF pathway also increased significantly (p < 0.05). The EsALF-3 and EsMyd88 were even significantly higher expressed in response to the second A. hydrophila challenge, but their expressions all decreased (p < 0.05) when EsTLR1 was knocked down by RNAi. After the naïve crabs received an injection with the recombinant protein of EsALF-1 (rEsALF-1) or EsALF-3 (rEsALF-3), their survival rate increased significantly (p < 0.05) upon A. hydrophila stimulation. In contrast, the survival rate of the primed crabs reduced significantly (p < 0.05) after they received an injection with the antibody of EsALF-1 or EsALF-3. The enhanced expressions of EsALF-1 and EsALF-3 after A. hydrophilap riming stimulation could sustain for four weeks. All the results suggested that the EsTLR1-mediated productions of EsALF-1 and EsALF-3 in haemolymph played an indispensable role in the month-long humoral immune protection induced by A. hydrophila, which provides solid evidence of immune priming in crabs and a valuable reference for further understanding immune memory in invertebrates.
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影响因子：
--
作者：
Christofi T;Apidianakis Y
通讯作者：
Apidianakis Y
影响因子：
2.9
作者：
Imler, Jean-Luc
通讯作者：
Imler, Jean-Luc
一种新的非吞噬细胞 TLR6，具有来自太平洋牡蛎巨牡蛎的广泛识别配体
影响因子：
2.9
作者：
Wang, Weilin;Zhang, Tao;Song, Linsheng
通讯作者：
Song, Linsheng
影响因子：
3.7
作者：
Lin YC;Chen JC;Morni WZ;Putra DF;Huang CL;Li CC;Hsieh JF
通讯作者：
Hsieh JF
DOI：
10.1126/science.1190689
发表时间：
2010-09-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rodrigues J;Brayner FA;Alves LC;Dixit R;Barillas-Mury C
通讯作者：
Barillas-Mury C