Vaccination enhances early immune responses in white shrimp Litopenaeus vannamei after secondary exposure to Vibrio alginolyticus.
Vaccination enhances early immune responses in white shrimp Litopenaeus vannamei after secondary exposure to Vibrio alginolyticus.
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DOI：
10.1371/journal.pone.0069722
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hsieh JF
中科院分区：
文献类型：
--
作者：
Lin YC;Chen JC;Morni WZ;Putra DF;Huang CL;Li CC;Hsieh JF
最近的研究表明，昆虫和虾中存在特异性记忆或某种形式的适应性免疫。高变模式识别分子，称为唐氏综合征细胞粘附分子，能够在无脊椎动物中进行特异性识别和免疫启动。本研究旨在了解白色凡纳滨对虾初次和二次暴露溶藻弧菌后的免疫应答模式。测定了经PE和SE处理的对虾对溶藻弧菌的免疫指标和造血组织增殖情况。在PE试验中，测量了接受热灭活的溶藻弧菌（HVa）和福尔马林灭活的溶藻弧菌（FVa）的虾的免疫参数和HPT的增殖。在SE至活的溶藻弧菌（LVa）后，测量7天-HVa-PE虾（在7天后接受初次暴露于HVa的虾）和7天-FVa-PE虾（在7天后接受初次暴露于FVa的虾）的死亡率、免疫参数和HPT的增殖。对7 ~ 35日龄HVa-PE和FVa-PE虾的吞噬活性和清除效率进行了检测。接受HVa的虾在第1天表现出较早的免疫应答增加，而接受FVa的虾在第5天表现出较晚的免疫应答增加。7天-FVa-PE虾在遭遇SE到LVa时显示出免疫增强，而7天-HVa-PE虾显示出免疫的轻微增强。7天-FVa-PE虾显示出更高的增殖和HPT有丝分裂指数。28天后，HVa-PE和FVa-PE虾的吞噬活性和清除率均保持较高。HVa和FVa接收虾显示细菌在被吞噬之前凝集。FVa作为疫苗发挥作用，而HVa作为诱导剂发挥作用，并可用作免疫佐剂。FVa和HVa的组合混合物可用作“疫苗组分”以调节虾的免疫力。
Recent work suggested that the presence of specific memory or some form of adaptive immunity occurs in insects and shrimp. Hypervariable pattern recognition molecules, known as Down syndrome cell adhesion molecules, are able to mount specific recognition, and immune priming in invertebrates. In the present study, we attempted to understand the immune response pattern of white shrimp Litopenaeus vannamei which received primary (PE) and secondary exposure (SE) to Vibrio alginolyticus. Immune parameters and proliferation of haematopoietic tissues (HPTs) of shrimp which had received PE and SE to V. alginolyticus were measured. In the PE trial, the immune parameters and proliferation of HPTs of shrimp that received heat-killed V. alginolyticus (HVa) and formalin-inactivated V. alginolyticus (FVa) were measured. Mortality, immune parameters and proliferation of HPTs of 7-day-HVa-PE shrimp (shrimp that received primary exposure to HVa after 7 days) and 7-day-FVa-PE shrimp (shrimp that received primary exposure to FVa after 7 days) following SE to live V. alginolyticus (LVa) were measured. Phagocytic activity and clearance efficiency were examined for the 7∼35-day-HVa-PE and FVa-PE shrimp. HVa-receiving shrimp showed an earlier increase in the immune response on day 1, whereas FVa-receiving shrimp showed a late increase in the immune response on day 5. The 7-day-FVa-PE shrimp showed enhancement of immunity when encountering SE to LVa, whereas 7-day-HVa-PE shrimp showed a minor enhancement in immunity. 7-day-FVa-PE shrimp showed higher proliferation and an HPT mitotic index. Both phagocytic activity and clearance maintained higher for both HVa-PE and FVa-PE shrimp after 28 days. HVa- and FVa-receiving shrimp showed the bacteria agglutinated prior to being phagocytised. FVa functions as a vaccine, whereas HVa functions as an inducer and can be used as an immune adjuvant. A combined mixture of FVa and HVa can serve as a “vaccine component” to modulate the immunity of shrimp.
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影响因子：
2.9
作者：
Chou, Pin-Hsiang;Chang, Hao-Shuo;Wang, K. C. Han-Ching
通讯作者：
Wang, K. C. Han-Ching
影响因子：
9.8
作者：
Dong, Yuemei;Taylor, Harry E;Dimopoulos, George
通讯作者：
Dimopoulos, George
影响因子：
16.8
作者：
Kurtz, J
通讯作者：
Kurtz, J
影响因子：
4.7
作者：
Chou, Pin-Hsiang;Chang, Hao-Shuo;Wang, K. C. Han-Ching
通讯作者：
Wang, K. C. Han-Ching
影响因子：
4.7
作者：
Hou, WY;Chen, JC
通讯作者：
Chen, JC