A hybrid scanning mode for fast scanning ion conductance microscopy (SICM) imaging.
A hybrid scanning mode for fast scanning ion conductance microscopy (SICM) imaging.
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DOI:
10.1016/j.ultramic.2012.06.015
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发表时间:
2012-10
期刊:
影响因子:
2.2
通讯作者:
Klenerman, David
中科院分区:
文献类型:
--
作者:
Zhukov, Alex;Richards, Owen;Ostanin, Victor;Korchev, Yuri;Klenerman, David
We have developed a new method of controlling the pipette for scanning ion conductance microscopy to obtain high-resolution images faster. The method keeps the pipette close to the surface during a single line scan but does not follow the exact surface topography, which is calculated by using the ion current. Using an FPGA platform we demonstrate this new method on model test samples and then on live cells. This method will be particularly useful to follow changes occurring on relatively flat regions of the cell surface at high spatial and temporal resolutions. ► We introduce a new method for fast scanning ion conductance microscopy (FSCIM). ► Method based on adaptive scanning using a field programmable gate array. ► Method gives improved sampling rate compared to other SICM control methods. ► Comparison of hopping mode SICM and FSICM made on live A6 cells.
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影响因子:
48
作者:
Novak, Pavel;Li, Chao;Shevchuk, Andrew I.;Stepanyan, Ruben;Caldwell, Matthew;Hughes, Simon;Smart, Trevor G.;Gorelik, Julia;Ostanin, Victor P.;Lab, Max J.;Moss, Guy W. J.;Frolenkov, Gregory I.;Klenerman, David;Korchev, Yuri E.
通讯作者:
Korchev, Yuri E.
DOI:
10.1016/j.pnsc.2008.01.011
发表时间:
2008-06-10
影响因子:
4.7
作者:
Li, Chao;Johnson, Nicholas;Klenerman, David
通讯作者:
Klenerman, David
影响因子:
4.1
作者:
Gorelik, J;Zhang, YJ;Korchev, YE
通讯作者:
Korchev, YE
影响因子:
3.4
作者:
Shevchuk, AI;Gorelik, J;Korchev, YE
通讯作者:
Korchev, YE
影响因子:
2.2
作者:
Pastré, D;Iwamoto, H;Shao, ZF
通讯作者:
Shao, ZF