Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes.
Differentiation of Bone Mesenchymal Stem Cells Into Vascular Endothelial Cell-Like Cells Using Functionalized Single-Walled Carbon Nanotubes.
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DOI：
10.3389/fbioe.2022.913080
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发表时间：
2022
影响因子：
5.7
通讯作者：
Wan, Qianbing
中科院分区：
文献类型：
--
作者：
Luo, Feng;Li, Ruyi;Zheng, Huaping;Xu, Yichen;Yang, Linxin;Qu, Changxing;Hong, Guang;Wan, Qianbing
关键词：
碳纳米管（Carbon nanotubes, CNTs）具有优异的力学和生物学性能，是一种很有前途的骨再生生物活性支架材料。血管化是骨组织工程的关键，而血管化不足是组织工程支架长期存在的问题。然而，CNTs对血管化的影响仍然很小。本研究对原始单壁碳纳米管（SWNTs）进行了纯化，制备了不同比例的SWNTs/EDC复合材料，并对其表面形貌和理化性质进行了研究。此外，通过诱导骨间充质干细胞（BMSCs）向血管内皮细胞样细胞（vec样细胞）分化，研究了swnt /EDC对血管形成的影响。结果表明，成功制备了SWNTs/EDC复合材料，EDC嵌入到SWNTs基体中并均匀分布在复合材料中。原子力显微镜（AFM）、红外光谱（FTIR）和拉曼光谱（Raman）结果证实了swcnts /EDC复合材料的形成。此外，SWNTs/EDC复合材料的表面形貌呈现出粗糙的表面，这可能会对细胞功能产生积极影响。体外细胞培养表明，单壁碳纳米管及其/EDC复合材料具有良好的生物相容性和生物活性。质量/体积比为1:10的swnt /EDC复合材料对骨髓间充质干细胞的增殖和分化促进效果最好。此外，swnt /EDC复合材料培养后，约78.3%±4.2%的培养细胞FITC-UEA-1和DiI-Ac-LDL双染色呈双阳性。此外，SWNTs/EDC复合组中代表性内皮细胞标志物VEGF、VEGF- r2、CD31和vWF的RNA表达显著高于对照组和SWNTs组。在我们研究的局限性下，结果表明SWNTs/EDC复合材料可以促进BMSCs向vec样细胞分化，并积极影响血管生成和骨再生。
Carbon nanotubes (CNTs) are a promising bioactive scaffold for bone regeneration because of their superior mechanical and biological properties. Vascularization is crucial in bone tissue engineering, and insufficient vascularization is a long-standing problem in tissue-engineered scaffolds. However, the effect of CNTs on vascularization is still minimal. In the current study, pristine single-walled carbon nanotubes (SWNTs) were purified to prepare different ratios of SWNTs/EDC composites, and their surface morphology and physicochemical properties of SWNTs/EDC were studied. Furthermore, the effect of SWNTs/EDC on vascularization was investigated by inducing the differentiation of bone mesenchymal stem cells (BMSCs) into vascular endothelial cell-like cells (VEC-like cells). Results showed that SWNTs/EDC composite was successfully prepared, and EDC was embedded in the SWNTs matrix and uniformly distributed throughout the composites. The AFM, FTIR spectra, and Raman results confirmed the formation of SWNTs/EDC composites. Besides, the surface topography of the SWNTs/EDC composites presents a rough surface, which may positively affect cell function. In vitro cell culture revealed that SWNTs and SWNTs/EDC composites exhibited excellent biocompatibility and bioactivity. The SWNTs/EDC composite at mass/volume ratios 1:10 had the best enhancement of proliferation and differentiation of BMSCs. Moreover, after culture with SWNTs/EDC composite, approximately 78.3% ± 4.2% of cultured cells are double-positive for FITC-UEA-1 and DiI-Ac-LDL double staining. Additionally, the RNA expression of representative endothelial cell markers VEGF, VEGF-R2, CD31, and vWF in the SWNTs/EDC composite group was significantly higher than those in the control and SWNTs group. With the limitation of our study, the results suggested that SWNTs/EDC composite can promote BMSCs differentiation into VEC-like cells and positively affect angiogenesis and bone regeneration.
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