The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic.
The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic.
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DOI：
10.1093/rb/rbx028
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发表时间：
2018-06
影响因子：
6.7
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Chen Y;Wang J;Zhu X;Chen X;Yang X;Zhang K;Fan Y;Zhang X
多孔磷酸钙（CaP）陶瓷的骨诱导性已被广泛研究和证实，其可能归因于植入陶瓷体内后血管网络的快速形成。本研究系统研究了骨诱导性多孔双相磷酸钙陶瓷（BCP）对骨髓间充质干细胞（BMSCs）迁移和分化的影响，以探讨CaP陶瓷内血管化的机制。结果表明，在transwell模型中，BMSCs可以向BCP陶瓷定向迁移，而预先在陶瓷表面接种巨噬细胞可以促进BMSCs的迁移。此外，在体内外生理条件下，BMSCs在BCP陶瓷片上直接培养，发现BMSCs在BCP陶瓷的刺激下发生向血管内皮细胞（vascular endothelial cells，VECs）的分化，VECs相关特征因子激酶插入结构域受体（kinase insert domain receptor，kinase insert domain receptor，vonwillebrand factor，血管细胞粘附分子-1和钙粘蛋白5。本研究为解释多孔CaP陶瓷体内植入后快速血管化过程中血管内皮细胞的来源提供了可能性，对揭示陶瓷血管化机制具有一定的指导意义。
Osteoinductivity of porous calcium phosphate (CaP) ceramics has been widely investigated and confirmed, and it might be attributed to the rapid formation of the vascular networks after in vivo implantation of the ceramics. In this study, to explore the vascularization mechanism within the CaP ceramics, the migration and differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) under the stimulation of porous biphasic calcium phosphate (BCP) ceramic with excellent osteoinductivity were systematically investigated. The results indicated that the directional migration of BMSCs toward BCP ceramic occurred when evaluated by using a transwell model, and the BMSCs migration was enhanced by the seeded macrophages on the ceramic in advance. Besides, by directly culturing BMSCs on BCP ceramic discs under both in vitro and in vivo physiological environment, it was found that the differentiation of BMSCs toward vascular endothelial cells (VECs) happened under the stimulation of BCP ceramic, as was confirmed by the up-regulated gene expressions and protein secretions of VECs-related characteristic factors, including kinase insert domain receptor, von willebrand factor, vascular cell adhesion molecule-1 and cadherin 5 in the BMSCs. This study offered a possibility for explaining the origin of VECs during the rapid vascularization process after in vivo implantation of porous CaP ceramics and could give some useful guidance to reveal the vascularization mechanism of the ceramics.
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影响因子：
14
作者：
Chen, Sulin;Jones, Jacqueline A.;Xu, Yongan;Low, Hong-Yee;Anderson, James M.;Leong, Kam W.
通讯作者：
Leong, Kam W.
DOI：
10.1007/s12306-008-0064-1
发表时间：
2008-12-01
期刊：
La Chirurgia degli organi di movimento
影响因子：
--
作者：
Devescovi, Valentina;Leonardi, Elisa;Cenni, Elisabetta
通讯作者：
Cenni, Elisabetta
影响因子：
12.7
作者：
Henkel J;Woodruff MA;Epari DR;Steck R;Glatt V;Dickinson IC;Choong PF;Schuetz MA;Hutmacher DW
通讯作者：
Hutmacher DW
影响因子：
0.1
作者：
Chen, Ying;Wang, Jing;Zhang, Xingdong
通讯作者：
Zhang, Xingdong
影响因子：
4.9
作者：
Habibovic, P;Sees, TM;de Groot, K
通讯作者：
de Groot, K