Evaluation of the inhibitory effects of vaginal microorganisms on sperm motility in vitro
Evaluation of the inhibitory effects of vaginal microorganisms on sperm motility in vitro
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阴道微生物对精子活力抑制作用的体外评价
DOI：
10.3892/etm.2019.8237
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发表时间：
2019-11
影响因子：
--
通讯作者：
陈廷涛
中科院分区：
文献类型：
--
作者：
陈廷涛
女性不孕症通常是由于衰老、身体损伤、激素紊乱和生活方式或环境因素造成的。然而，乳杆菌在女性不孕症中的潜在作用在很大程度上仍未被探索。本研究采用高通量测序、实时荧光PCR、细菌粘附试验和精子活力试验等方法，对精子细菌、阴道细菌和与精子共培养的阴道细菌的微生物多样性、粘附特性以及对精子活力的影响进行了研究。结果表明，在共培养组中，乳酸杆菌粘附于精子细胞的数量是对照种肠球菌的332倍，并且使用京都基因和基因组百科全书进行的测序数据分析表明，粘附的微生物降低了精子细胞的活力。将阴道分离株以及用作对照的细菌菌株与精子共培养，结果表明所有菌株都能够大量粘附于精子细胞。益生菌乳酸杆菌（L.）菌株L. crispatus，L.嗜酸乳杆菌L. helveticus和L. gasseri显著降低精子活动力（基于一般、直接和非直接前向运动力、总运动力和平均路径速度的测量; P<0.05）。此外，L. crispatus，L.嗜酸乳杆菌L.唾液腺L. helveticus和L. Gasseri显著降低精子在粘性介质中的穿透。基于这些结果，可以假设乳酸菌对精子活力的减弱作用可能有利于健康夫妇防止异常精子和卵子的结合，但可能不利于严重弱精子症、少精子症或无精子症的男性。
Female infertility usually occurs as a result of ageing, physical impairment, hormone disturbances and lifestyle or environmental factors. However, the potential role of Lactobacillus in female infertility has remained largely unexplored. In the present study, high-throughput sequencing, real-time PCR, bacterial adherence assays and sperm motility assays were used to evaluate the microbial diversity, adherence properties and effect on sperm motility of sperm bacteria, vaginal bacteria and vaginal bacteria that had been co-cultured with sperm. The results indicated that in the co-culture group, Lactobacillus adhered to sperm cells in numbers that were 332-fold higher than those of control species Enterococcus and analysis of sequencing data using the Kyoto Encyclopedia of Genes and Genomes indicated that adhered microbes reduced sperm cell motility. Vaginal isolates, as well as bacterial strains used as controls, were co-cultured with sperm and it was indicated that all strains were able to adhere to sperm cells in large numbers. The probiotic Lactobacillus (L.) strains L. crispatus, L. acidophilus, L. helveticus and L. gasseri significantly reduced sperm motility (based on measurements of general, straightforward and non-straightforward progressive motility, total motility and average path velocity; P<0.05). Furthermore, L. crispatus, L. acidophilus, L. salivarius, L. helveticus and L. gasseri markedly reduced sperm penetration in a viscous medium. Based on these results, it may be hypothesized that the weakening effect of Lactobacillus on sperm motility may be beneficial for healthy couples to prevent the combination of abnormal sperms and eggs, but may be detrimental for males with severe asthenospermia, oligospermia or aspermia.
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影响因子：
2.9
作者：
Charlotte Jones
通讯作者：
Charlotte Jones
乳酸菌作为生物标志物和制剂，可以促进阴道健康的各个方面。
影响因子：
4
作者：
Petrova MI;Lievens E;Malik S;Imholz N;Lebeer S
通讯作者：
Lebeer S
梗阻性和非梗阻性无精症患者的精液细菌组成
影响因子：
2.7
作者：
Chen H;Luo T;Chen T;Wang G
通讯作者：
Wang G
影响因子：
2.4
作者：
G. Dohle
通讯作者：
G. Dohle
影响因子：
2.6
作者：
Maendar, Reet;Punab, Margus;Truu, Jaak
通讯作者：
Truu, Jaak