Mouse current vocalization threshold measured with a neurospecific nociception assay: the effect of sex, morphine, and isoflurane.
Mouse current vocalization threshold measured with a neurospecific nociception assay: the effect of sex, morphine, and isoflurane.
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DOI：
10.1016/j.jneumeth.2011.08.011
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发表时间：
2011-10-15
影响因子：
3
通讯作者：
Quezado ZM
中科院分区：
文献类型：
--
作者：
Spornick N;Guptill V;Koziol D;Wesley R;Finkel J;Quezado ZM
最近，在用于评价感觉神经元的设备中增加了频率为2000、250和5 Hz的正弦波电刺激，分别用于评价A β、A δ和C感觉神经元。我们开发了一种自动化的伤害性试验，使用正弦波刺激方法来确定当前的发声阈值响应于2000，250和5赫兹，并检查性别，镇痛药和麻醉剂在小鼠中的影响。基线时，在2000、250和5 Hz下，雄性小鼠的平均电流发声阈值明显高于雌性小鼠（p ≤ 0.019）。鞘内注射吗啡后1小时，当前发声阈值百分比变化较基线显著增加，其随剂量（p = 0.0001）和使用频率（p <0.0001）而变化。具体而言，随着吗啡剂量的增加，与250和2000 Hz刺激相比，响应于5 Hz的电流发声阈值百分比变化从基线显著增加，呈显著有序模式：5 Hz> 250 Hz（p <0.0001）和250 Hz> 2000 Hz（p = 0.0002）。暴露后45分钟，异氟烷对任何频率下的电流发声阈值均无影响。因此，我们的研究结果表明，在小鼠中使用正弦波刺激的这种自动化伤害感受测定对于测量性别、阿片类药物和麻醉剂对体内优先刺激A β、A δ和C感觉纤维的电刺激的反应的影响可能是有价值的。这项调查表明，该试验的验证，并支持其用于检查小鼠的伤害感受机制。
Sine-wave electrical stimulation at frequencies 2000, 250, and 5 Hz to respectively evaluate Aβ, Aδ, and C sensory neurons has recently been added to the armamentarium used to evaluate sensory neurons. We developed an automated nociception assay using sine-wave stimulation methodology to determine current vocalization threshold in response to 2000, 250, and 5 Hz and examine the effects of sex, analgesics, and anesthetics in mice. At baseline, males had significantly higher mean current vocalization thresholds compared with female mice at 2000, 250, and 5 Hz (p ≤ 0.019). By 1 h after intrathecal injections of morphine there were significant increases in current vocalization threshold percent changes from baseline that varied with doses (p = 0.0001) and frequency used (p < 0.0001). Specifically, with increasing doses of morphine, there were significantly greater increases in current vocalization threshold percent changes from baseline in response to 5 Hz compared with 250 and 2000 Hz stimulation in a significantly ordered pattern: 5 Hz > 250 Hz (p < 0.0001) and 250 Hz > 2000 Hz (p = 0.0002). Forty-five minutes after exposure, there were no effects of isoflurane on current vocalization thresholds at any frequency. Therefore, our findings suggest that this automated nociception assay using sine-wave stimulation in mice, can be valuable for measurements of the effects of sex, opioids, and anesthetics on the response to electrical stimuli that preferentially stimulate Aβ, Aδ, and C-sensory fibers in vivo. This investigation suggests the validation of this assay and supports its use to examine mechanisms of nociception in mice.
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