Effect of iRoot Fast Set root repair material on the proliferation, migration and differentiation of human dental pulp stem cells in vitro.
Effect of iRoot Fast Set root repair material on the proliferation, migration and differentiation of human dental pulp stem cells in vitro.
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iRoot Fast Set牙根修复材料对人牙髓干细胞体外增殖、迁移和分化的影响
DOI：
10.1371/journal.pone.0186848
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Sun Y;Luo T;Shen Y;Haapasalo M;Zou L;Liu J
本研究探讨了iRootFastSet根管修复材料（iRootFS）对人牙髓干细胞（hDPSCs）增殖、迁移和分化的影响。用浓度为0.2和2 mg/mL的iRoot FS洗脱液（分别称为FS 0.2和FS 2）和相应浓度的Biodentine（BD; Septodont，Saint毛尔des Faussés，法国）洗脱液处理hDPSC作为阳性对照。进行CCK 8测定以确定细胞增殖。进行伤口愈合和transwell测定以检查细胞迁移。通过碱性磷酸酶活性、茜素红S染色和实时定量逆转录聚合酶链反应（qRT-PCR）分析分化基因标志物的mRNA表达来评价成骨分化。FS组和BD组在第3天和第7天的细胞增殖高于空白对照组。FS0.2能促进hDPSCs的迁移和成骨分化。这些结果表明，iRoot FS是一种促进hDPSCs增殖、迁移和成骨分化的生物活性材料。
The present study investigated the effect of iRoot Fast Set root repair material (iRoot FS) on the proliferation, migration and differentiation of human dental pulp stem cells (hDPSCs). The hDPSCs were treated with eluates of iRoot FS at concentrations of 0.2 and 2 mg/mL, referred to as FS0.2 and FS2, respectively, and Biodentine (BD; Septodont, Saint Maur des Faussés, France) eluates at the corresponding concentrations as positive controls. A CCK8 assay was performed to determine cell proliferation. Wound healing and transwell assays were conducted to examine cell migration. Osteogenic differentiation was evaluated based on alkaline phosphatase activity, Alizarin Red S staining and quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) to analyze the mRNA expression of differentiation gene markers. Cell proliferation was higher in the FS and BD groups than in the blank controls at 3 and 7 days. Moreover, FS0.2 enhanced cell migration and significantly promoted the osteogenic differentiation of hDPSCs. These findings suggested that iRoot FS is a bioactive material that promotes the proliferation, migration and osteogenic differentiation of hDPSCs.
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影响因子：
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通讯作者：
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影响因子：
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作者：
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