A naturally occurring conditional albino mutant in rice caused by defects in the plastid-localized OsABCI8 transporter
A naturally occurring conditional albino mutant in rice caused by defects in the plastid-localized OsABCI8 transporter
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水稻中自然发生的条件性白化突变体，由质体定位的 OsABCI8 转运蛋白缺陷引起
DOI：
10.1007/s11103-017-0598-4
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发表时间：
2017-03
影响因子：
5.1
通讯作者：
Zhang Xiang-Qian
中科院分区：
文献类型：
--
作者：
Zeng Xiuyu;Tang Ran;Guo Herong;Ke Shanwen;Teng Bin;Hung Yu-Hung;Xu Zhenjiang;Xie Xin-Ming;Hsieh Tzung-Fu;Zhang Xiang-Qian
ATP结合盒（ABC）转运蛋白可跨膜转运多种分子。植物中存在一系列与原核多亚基ABC转运蛋白相似的ABC蛋白，称为ABC Ⅰ组。然而，它们中的大多数的功能还没有被很好地理解。在此，我们描述了一个自然发生的水稻突变体，该突变体在田间连续阴雨天下表现出白化病表型，但在雨季后逐渐恢复到正常的绿色。分子和遗传学分析表明，这些表型是由编码ABCI家族成员的OsABCI 8突变引起的。亚细胞定位表明OsABCI 8是一个叶绿体ABC转运蛋白。OsABCI 8的表达在雨天比晴天显著增强。除了叶绿体发育和叶绿素生物合成的缺陷外，突变体表型还伴随着更高的铁积累，表明OsABCI 8参与水稻中的铁运输和/或稳态。我们的研究结果表明，OsABCI 8代表一个保守的ABCI蛋白参与过渡金属的运输和/或稳态，并建议一个重要的作用，质体定位OsABCI 8叶绿体发育。
A wide range of molecules are transported across membranes by the ATP binding cassette (ABC) transporters. Plants possess a collection of ABC proteins bearing similarities to the components of prokaryotic multi subunit ABC transporters, designed as ABC group I. However the functions of most of them are not well understood. Here, we characterized a naturally occurring rice mutant that exhibited albino phenotype under continuous rainy days in the field, but gradually recovered to normal green after the rainy season. Molecular and genetic analyses revealed that the phenotypes were caused by a mutation in theOsABCI8that encoded a member of the ABCI family. Subcellular localization demonstrated that OsABCI8 is a chloroplast ABC transporter. Expression ofOsABCI8is significantly enhanced in rainy days compared to sunny days. Besides defects in chloroplast development and chlorophyll biosynthesis, the mutant phenotype is accompanied by a higher accumulation of iron, suggesting that OsABCI8 is involved in iron transportation and/or homeostasis in rice. Our results demonstrate that OsABCI8 represents a conserved ABCI protein involved in transition metals transportation and/or homeostasis and suggest an important role of the plastid-localized OsABCI8 for chloroplast development.
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DOI：
10.1073/pnas.0708367105
发表时间：
2008-07-29
影响因子：
11.1
作者：
Jeong, Jeeyon;Cohu, Christopher;Guerinot, Mary Lou
通讯作者：
Guerinot, Mary Lou
影响因子：
6.4
作者：
DEI, M
通讯作者：
DEI, M
影响因子：
7.4
作者：
B. Waters;D. Blevins;D. Eide
通讯作者：
B. Waters;D. Blevins;D. Eide
影响因子：
4.3
作者：
Zhou, Kunneng;Ren, Yulong;Wan, Jianmin
通讯作者：
Wan, Jianmin
杂交水稻（Oryza sativa L. ssp. indica）叶色基因 Ygl7 的定位克隆及功能分析。
影响因子：
3.7
作者：
Deng XJ;Zhang HQ;Wang Y;He F;Liu JL;Xiao X;Shu ZF;Li W;Wang GH;Wang GL
通讯作者：
Wang GL