Progressive structural and biomechanical changes in elastin degraded aorta.
Progressive structural and biomechanical changes in elastin degraded aorta.
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DOI：
10.1007/s10237-012-0404-9
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发表时间：
2013-04
影响因子：
3.5
通讯作者：
Zhang, Yanhang
中科院分区：
文献类型：
--
作者：
Chow, Ming-Jay;Mondonedo, Jarred R.;Johnson, Victor M.;Zhang, Yanhang
主动脉瘤是一种重要的临床疾病，其特征是常见的结构变化，如弹性蛋白的降解，平滑肌细胞的丢失，纤维状胶原的沉积增加。为了研究动脉的力学行为与结构/生化成分之间的关系，本研究使用了一个简单的动脉瘤化学降解模型，研究了动脉瘤的力学性质的渐进性变化。猪胸主动脉在温和的纯化弹性酶溶液(5U/mL)中消化6、12、24、48和96h。初步尺寸测量显示，在没有周期性负荷的情况下，弹性蛋白结构的破坏导致动脉扩张增加。用双向拉伸测试装置测量的消化动脉的机械性能经历了四个不同的阶段，即(1)初始软化阶段，(2)弹性体样，(3)可伸展但僵硬的阶段，和(4)胶原样支架。虽然阶段1、3和4预计是弹性蛋白降解的结果，但由于酶消化导致的S形状的主动脉应力-应变行为之前还没有报道。我们的结果表明，动脉弹性蛋白结构的逐渐变化可以通过不同的力学性质导致进展，从而揭示了在动脉瘤形成过程中可能存在一个重要的过渡阶段，这可能有助于动脉扩张。
Aortic aneurysm is an important clinical condition characterized by common structural changes such as the degradation of elastin, loss of smooth muscle cells, and increased deposition of fibrillary collagen. With the goal of investigating the relationship between the mechanical behavior and the structural/biochemical composition of an artery, this study used a simple chemical degradation model of aneurysm and investigated the progressive changes in mechanical properties. Porcine thoracic aortas were digested in a mild solution of purified elastase (5U/mL) for 6, 12, 24, 48, and 96 h. Initial size measurements show that disruption of the elastin structure leads to increased artery dilation in the absence of periodic loading. The mechanical properties of the digested arteries, measured with a biaxial tensile testing device, progress through four distinct stages termed (1) initial-softening, (2) elastomer-like, (3) extensible-but-stiff, and (4) collagen-scaffold-like. While stages 1, 3, and 4 are expected as a result of elastin degradation, the S-shaped stress versus strain behavior of the aorta resulting from enzyme digestion has not been reported previously. Our results suggest that gradual changes in the structure of elastin in the artery can lead to a progression through different mechanical properties and thus reveal the potential existence of an important transition stage that could contribute to artery dilation during aneurysm formation.
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影响因子：
2.4
作者：
Humphrey, J. D.;Eberth, J. F.;Dye, W. W.;Gleason, R. L.
通讯作者：
Gleason, R. L.
影响因子：
37.8
作者：
Isenburg, Jason C.;Simionescu, Dan T.;Vyavahare, Narendra R.
通讯作者：
Vyavahare, Narendra R.
影响因子：
4.3
作者：
Gasser, T. Christian;Gorgulu, Goray;Swedenborg, Jesper
通讯作者：
Swedenborg, Jesper
影响因子：
--
作者：
COX, RH
通讯作者：
COX, RH
影响因子：
15.9
作者：
GERTZ, SD;KURGAN, A;EISENBERG, D
通讯作者：
EISENBERG, D