Resistance loci affecting distinct stages of fungal pathogenesis: use of introgression lines for QTL mapping and characterization in the maize--Setosphaeria turcica pathosystem.
Resistance loci affecting distinct stages of fungal pathogenesis: use of introgression lines for QTL mapping and characterization in the maize--Setosphaeria turcica pathosystem.
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DOI：
10.1186/1471-2229-10-103
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发表时间：
2010-06-08
影响因子：
5.3
通讯作者：
Nelson RJ
中科院分区：
文献类型：
--
作者：
Chung CL;Longfellow JM;Walsh EK;Kerdieh Z;Van Esbroeck G;Balint-Kurti P;Nelson RJ
对一系列致病系统中宿主-病原体相互作用的研究揭示了植物降低致病效率的一系列机制。虽然R基因介导的抗性赋予针对病原体入侵的高效防御反应，但数量抗性与减少疾病进展的中等水平的抗性相关。为了验证特定基因座影响真菌致病的不同阶段的假设，一组玉米渐渗系用于定位和表征调节对北方叶枯病（NLB）的致病因子玉米大斑病菌（Setosphaeria turcica）的抗性的数量性状基因座（QTL）。为了更好地理解数量抗性的性质，进一步测试了鉴定的QTL的三个次要假设：（1）病害QTL随宿主发育阶段而不同;（2）它们的表现随环境而变化;以及（3）它们制约广谱抗性。在一组82个渐渗系中，7个系被证实比B73更抗或更感。在BC 4F 2分离群体和高渗系中验证了2个NLB QTL。这些位点，指定qNLB 1.02和qNLB 1.06，通过比较渗入系与B73的一系列宏观和微观疾病组分针对不同阶段的NLB发展进行了详细研究。重复的温室和田间试验表明，qNLB 1.06Tx303（Tx 303等位基因在bin 1.06）降低真菌渗透的效率，而qNLB 1.02B73（B73等位基因在bin 1.02）增强胼胝质和酚类物质的积累感染部位周围，减少菌丝生长到维管束和损害随后的坏死营养型定殖在叶片中。QTL在幼龄和成年植株中同样有效; qNLB 1.06Tx303在田间表现出比在温室中更大的有效性。qNLB 1.02 B73除了对NLB抗性外，还对枯萎病和锈病具有抗性，而qNLB 1.06 Tx 303对枯萎病具有抗性。非特异性耐药可能是由于多效性或连锁。我们的研究已经成功鉴定了两个可靠表达的QTL，它们可能被用来保护玉米免受S。土耳其在不同的环境。这种鉴定和解剖植物数量抗性的方法将有助于数量抗性在作物保护中的应用。
Studies on host-pathogen interactions in a range of pathosystems have revealed an array of mechanisms by which plants reduce the efficiency of pathogenesis. While R-gene mediated resistance confers highly effective defense responses against pathogen invasion, quantitative resistance is associated with intermediate levels of resistance that reduces disease progress. To test the hypothesis that specific loci affect distinct stages of fungal pathogenesis, a set of maize introgression lines was used for mapping and characterization of quantitative trait loci (QTL) conditioning resistance to Setosphaeria turcica, the causal agent of northern leaf blight (NLB). To better understand the nature of quantitative resistance, the identified QTL were further tested for three secondary hypotheses: (1) that disease QTL differ by host developmental stage; (2) that their performance changes across environments; and (3) that they condition broad-spectrum resistance. Among a set of 82 introgression lines, seven lines were confirmed as more resistant or susceptible than B73. Two NLB QTL were validated in BC4F2 segregating populations and advanced introgression lines. These loci, designated qNLB1.02 and qNLB1.06, were investigated in detail by comparing the introgression lines with B73 for a series of macroscopic and microscopic disease components targeting different stages of NLB development. Repeated greenhouse and field trials revealed that qNLB1.06Tx303 (the Tx303 allele at bin 1.06) reduces the efficiency of fungal penetration, while qNLB1.02B73 (the B73 allele at bin 1.02) enhances the accumulation of callose and phenolics surrounding infection sites, reduces hyphal growth into the vascular bundle and impairs the subsequent necrotrophic colonization in the leaves. The QTL were equally effective in both juvenile and adult plants; qNLB1.06Tx303 showed greater effectiveness in the field than in the greenhouse. In addition to NLB resistance, qNLB1.02B73 was associated with resistance to Stewart's wilt and common rust, while qNLB1.06Tx303 conferred resistance to Stewart's wilt. The non-specific resistance may be attributed to pleiotropy or linkage. Our research has led to successful identification of two reliably-expressed QTL that can potentially be utilized to protect maize from S. turcica in different environments. This approach to identifying and dissecting quantitative resistance in plants will facilitate the application of quantitative resistance in crop protection.
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影响因子：
2
作者：
BROWN, AHD;MUNDAY, J;ORAM, RN
通讯作者：
ORAM, RN
影响因子：
4.5
作者：
CARSON, ML
通讯作者：
CARSON, ML
影响因子：
3.1
作者：
Collins, A;Milbourne, D;Waugh, R
通讯作者：
Waugh, R
影响因子：
3.2
作者：
BREWSTER, VA;CARSON, ML;WICKS, ZW
通讯作者：
WICKS, ZW
影响因子：
30.8
作者：
Consonni, Chiara;Humphry, Matthew E.;Panstruga, Ralph
通讯作者：
Panstruga, Ralph