CHARACTERIZING CALCIUM INFLUX VIA VOLTAGE- AND LIGAND-GATED CALCIUM CHANNELS IN EMBRYONIC ALLIGATOR NEURONS IN CULTURE.
CHARACTERIZING CALCIUM INFLUX VIA VOLTAGE- AND LIGAND-GATED CALCIUM CHANNELS IN EMBRYONIC ALLIGATOR NEURONS IN CULTURE.
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DOI：
10.2478/s13380-013-0132-3
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发表时间：
2013-09-01
影响因子：
2.1
通讯作者：
Khanna R
中科院分区：
文献类型：
--
作者：
Ju W;Wu J;Pritz MB;Khanna R
脊椎动物的大脑有许多共同的特征。在发育的早期，后脑和间脑的构造是相似的。只是在后来的时间里，形态上的差异才出现。可能影响这种变化的因素包括神经元的某些生理特性。作为研究这一问题的第一步，培养了胚胎短吻鳄脑神经元，并对其钙反应进行了表征。本报告是第一个记录的人工脑脊液（ACSF），以及在标准的哺乳动物组织培养基中补充生长因子的鳄鱼脑神经元的文化。短吻鳄脑神经元培养物存活至少1周，24小时出现单极神经突。采用Fura-2 AM，在这些神经元中观察到强去极化诱导的钙内流。使用电压门控钙通道的选择性阻断剂，在这些神经元中的N-，P/Q-，R-，T-和L-型通道的贡献进行了评估，并记录其存在。最后，用兴奋性毒性刺激（谷氨酸+甘氨酸）对短吻鳄脑神经元进行了挑战，其中经典的NMDA受体拮抗剂可以防止延迟的钙失调。
Vertebrate brains share many features in common. Early in development, both the hindbrain and diencephalon are built similarly. Only later in time do differences in morphology occur. Factors that could potentially influence such changes include certain physiological properties of neurons. As an initial step to investigate this problem, embryonic Alligator brain neurons were cultured and calcium responses were characterized. The present report is the first to document culture of Alligator brain neurons in artificial cerebrospinal fluid (ACSF) as well as in standard mammalian tissue culture medium supplemented with growth factors. Alligator brain neuron cultures were viable for at least 1 week with unipolar neurites emerging by 24 hours. Employing Fura-2 AM, robust depolarization-induced calcium influx, was observed in these neurons. Using selective blockers of the voltage-gated calcium channels, the contributions of N-, P/Q-, R-, T-, and L-type channels in these neurons were assessed and their presence documented. Lastly, Alligator brain neurons were challenged with an excitotoxic stimulus (glutamate + glycine) where delayed calcium deregulation could be prevented by a classical NMDA receptor antagonist.
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影响因子：
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