Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods.
Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods.
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DOI：
10.1093/jxb/ert216
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发表时间：
2013-09
影响因子：
6.9
通讯作者：
Rintamäki E
中科院分区：
文献类型：
--
作者：
Lepistö A;Pakula E;Toivola J;Krieger-Liszkay A;Vignols F;Rintamäki E
质体定位的NADPH依赖性硫氧还蛋白还原酶C（NTRC）是一种独特的NTR酶，在单个多肽中含有还原酶和硫氧还蛋白结构域。拟南芥NTRC基因敲除株系（ntrc）表现出生长迟缓，特别是在短日照（SD）光周期下。本研究确定了叶绿体的过程，占生长减少SD驯化ntrc。最强的减少ntrc的增长发生在光周期与夜间长于14小时，而敲除的NTRC基因并没有改变拟南芥的昼夜节律钟控制的增长。缺乏NTRC调节叶绿体活性氧（ROS）代谢，但氧化应激不是主要原因，生长迟缓的SD驯化的NTRC。淀粉积累的缺乏使ntrc叶片特别容易受到长夜光周期的影响。酵母双杂交分析证实了NTRC与淀粉合成关键酶ADP-葡萄糖焦磷酸化酶的直接相互作用。ntrc系在光照期不能使淀粉合成最大化，这在SD条件下特别有害。拟南芥适应SD条件还涉及诱导上升的ROS生产的照明叶绿体不平衡的质体抗氧化系统的激活。有人提出，敲除NTRC挑战氧化还原调节淀粉合成，导致生长发育不良的突变株系适应SD光周期。
Plastid-localized NADPH-dependent thioredoxin reductase C (NTRC) is a unique NTR enzyme containing both reductase and thioredoxin domains in a single polypeptide. Arabidopsis thaliana NTRC knockout lines (ntrc) show retarded growth, especially under short-day (SD) photoperiods. This study identified chloroplast processes that accounted for growth reduction in SD-acclimated ntrc. The strongest reduction in ntrc growth occurred under photoperiods with nights longer than 14h, whereas knockout of the NTRC gene did not alter the circadian-clock-controlled growth of Arabidopsis. Lack of NTRC modulated chloroplast reactive oxygen species (ROS) metabolism, but oxidative stress was not the primary cause of retarded growth of SD-acclimated ntrc. Scarcity of starch accumulation made ntrc leaves particularly vulnerable to photoperiods with long nights. Direct interaction of NTRC and ADP-glucose pyrophosphorylase, a key enzyme in starch synthesis, was confirmed by yeast two-hybrid analysis. The ntrc line was not able to maximize starch synthesis during the light period, which was particularly detrimental under SD conditions. Acclimation of Arabidopsis to SD conditions also involved an inductive rise of ROS production in illuminated chloroplasts that was not counterbalanced by the activation of plastidial anti-oxidative systems. It is proposed that knockout of NTRC challenges redox regulation of starch synthesis, resulting in stunted growth of the mutant lines acclimated to the SD photoperiod.
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DOI：
10.1073/pnas.0406674102
发表时间：
2005-02-01
影响因子：
11.1
作者：
Mikkelsen, R;Mutenda, KE;Blennow, A
通讯作者：
Blennow, A
影响因子：
11.6
作者：
Kirchsteiger, Kerstin;Ferrandez, Julia;Javier Cejudo, Francisco
通讯作者：
Javier Cejudo, Francisco
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
6.9
作者：
Mubarakshina, Maria M.;Ivanov, Boris N.;Krieger-Liszkay, Anja
通讯作者：
Krieger-Liszkay, Anja
影响因子：
4.1
作者：
Kangasjarvi, Saijaliisa;Lepisto, Anna;Rintamaki, Eevi
通讯作者：
Rintamaki, Eevi