Parallel epigenomic and transcriptomic responses to viral infection in honey bees (Apis mellifera).
Parallel epigenomic and transcriptomic responses to viral infection in honey bees (Apis mellifera).
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DOI：
10.1371/journal.ppat.1004713
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发表时间：
2015-03
期刊：
影响因子：
6.7
通讯作者：
Grozinger C
中科院分区：
文献类型：
--
作者：
Galbraith DA;Yang X;Niño EL;Yi S;Grozinger C
世界各地的蜜蜂数量都在下降，美国养蜂人每年冬天都会失去 30% 的蜂群。尽管造成这些菌落损失的因素有多种，但越来越明显的是，病毒发挥了主要作用。然而，有关蜜蜂抗病毒免疫分子机制的信息却非常有限。在这里，我们研究了工蜂对病毒感染的转录和表观遗传（DNA 甲基化）反应。一天大的工蜂被喂食含有以色列急性麻痹病毒（IAPV）的溶液，这种病毒会导致肌肉麻痹和死亡，此前曾被认为与蜂群损失有关。感染后 20-24 小时内收集未感染的对照蜜蜂和感染的有症状蜜蜂。收集工人脂肪体进行分析，这是参与新陈代谢、解毒和免疫反应的主要组织。我们对工人脂肪体进行了转录组和亚硫酸氢盐测序，以确定与病毒感染相关的全基因组基因表达和 DNA 甲基化模式。与对照蜜蜂相比，受感染蜜蜂有 753 个差异表达基因（FDR<0.05），其中包括几个涉及表观遗传和抗病毒途径的基因。感染导致 156 个基因 (FDR<0.1) 的 DNA 甲基化状态发生显着变化，其中包括那些参与人类抗病毒反应的基因。显着差异表达基因和显着差异甲基化基因之间没有显着重叠，事实上，这些基因组的基因组特征非常不同。我们的结果表明，蜜蜂有两种不同的分子途径，由转录和甲基化介导，调节蛋白质水平和/或功能以应对病毒感染。蜜蜂是多种农作物的重要授粉者，但养蜂人每年都会遭受蜂群的严重损失。有几个因素与菌落损失有关，特别是以色列急性麻痹病毒（IAPV）感染。尽管病毒对蜜蜂健康很重要，但我们对介导宿主与病原体相互作用的分子机制的了解仍然有限。在这里，我们描述了蜜蜂对短期（<24 小时）IAPV 感染的表观基因组和转录组反应。我们发现受感染蜜蜂和对照蜜蜂之间 753 个基因存在显着的表达差异，包括涉及免疫和表观遗传途径的基因。然而，IAPV 调节的基因与对细菌或微孢子虫感染做出反应的基因没有显着重叠，这表明蜜蜂利用不同的途径来应对不同的免疫挑战。此外，156 个基因的 DNA 甲基化状态发生了显着变化，其中包括一些与人类抗病毒免疫反应有关的基因。有趣的是，差异甲基化基因和差异表达基因之间没有显着重叠，这表明蜜蜂可能具有平行的基因组机制来应对病毒感染。
Populations of honey bees are declining throughout the world, with US beekeepers losing 30% of their colonies each winter. Though multiple factors are driving these colony losses, it is increasingly clear that viruses play a major role. However, information about the molecular mechanisms mediating antiviral immunity in honey bees is surprisingly limited. Here, we examined the transcriptional and epigenetic (DNA methylation) responses to viral infection in honey bee workers. One-day old worker honey bees were fed solutions containing Israeli Acute Paralysis Virus (IAPV), a virus which causes muscle paralysis and death and has previously been associated with colony loss. Uninfected control and infected, symptomatic bees were collected within 20–24 hours after infection. Worker fat bodies, the primary tissue involved in metabolism, detoxification and immune responses, were collected for analysis. We performed transcriptome- and bisulfite-sequencing of the worker fat bodies to identify genome-wide gene expression and DNA methylation patterns associated with viral infection. There were 753 differentially expressed genes (FDR<0.05) in infected versus control bees, including several genes involved in epigenetic and antiviral pathways. DNA methylation status of 156 genes (FDR<0.1) changed significantly as a result of the infection, including those involved in antiviral responses in humans. There was no significant overlap between the significantly differentially expressed and significantly differentially methylated genes, and indeed, the genomic characteristics of these sets of genes were quite distinct. Our results indicate that honey bees have two distinct molecular pathways, mediated by transcription and methylation, that modulate protein levels and/or function in response to viral infections. Honey bees are a critical pollinator of a wide variety of agricultural crops, but beekeepers experience heavy annual losses of honey bee colonies. Several factors are associated with colony losses, in particular infection with Israeli Acute Paralysis Virus (IAPV). Despite the importance of viruses to honey bee health, our understanding of the molecular mechanisms mediating host-pathogen interactions is limited. Here, we characterized the epigenomic and transcriptomic responses of honey bees to short term (<24 hour) IAPV infection. We found significant expression differences in 753 genes between infected and control bees, including genes involved in immune and epigenetic pathways. However, IAPV-regulated genes did not overlap significantly with genes that respond to bacterial or microsporidian infection, suggesting that honey bees utilize distinct pathways for different immune challenges. Additionally, DNA methylation status of 156 genes changed significantly, including several genes that have been linked to antiviral immune responses in humans. Interestingly, there was no significant overlap between the differentially methylated and differentially expressed genes, suggesting that honey bees may possess a parallel genomic mechanisms to respond to viral infection.
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影响因子：
3.7
作者：
Cornman RS;Tarpy DR;Chen Y;Jeffreys L;Lopez D;Pettis JS;vanEngelsdorp D;Evans JD
通讯作者：
Evans JD
蜜蜂转录组的表观遗传调控：揭示甲基化基因的本质。
影响因子：
4.4
作者：
Foret S;Kucharski R;Pittelkow Y;Lockett GA;Maleszka R
通讯作者：
Maleszka R
DOI：
10.1073/pnas.96.8.4482
发表时间：
1999-04-13
影响因子：
11.1
作者：
Duret, L;Mouchiroud, D
通讯作者：
Mouchiroud, D
影响因子：
2.2
作者：
DeGrandi-Hoffman, Gloria;Chen, Yanping;Huang, Ming Hua
通讯作者：
Huang, Ming Hua
影响因子：
5.4
作者：
Ehrlich, Lorna S.;Medina, Gisselle N.;Carter, Carol A.
通讯作者：
Carter, Carol A.