Improved flow field-flow fractionation system applied to water-soluble polymers: programming, outlet stream splitting, and flow optimization.
Improved flow field-flow fractionation system applied to water-soluble polymers: programming, outlet stream splitting, and flow optimization.
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改进的流场流分馏系统应用于水溶性聚合物：编程、出口流分裂和流量优化。
DOI：
10.1021/ac00294a018
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发表时间：
1986
影响因子：
7.4
通讯作者：
Giddings,JC
中科院分区：
文献类型：
--
作者：
Wahlund,KG;Winegarner,HS;Caldwell,KD;Giddings,JC
本文报道了流场-流动分离（FFF）系统优化的几个新进展。这些发展包括现场编程的应用程序流FFF，一个新的出口分流器的设计和应用，并实现分离使用的流量大致一个数量级高于以前采用的流FFF。它示出了如何在这些发展援助的整体性能的优化，测量分辨率，速度，样品的可检测性，并避免过载。最后给出了磺化聚苯乙烯样品的实验结果，以说明这一新的发展。在被称为场流分离（FFF）（1-5）的大分子分离/表征方法家族中，流动FFF可能是应用最广泛的。它只要求样品可溶于或可分散于某种载液中，并能找到一种能保留样品的半透膜。分离是基于扩散系数的差异，其与分子量的关系通常很好地表征。借助FFF理论，可以直接从复杂样品分离后的洗脱曲线中获得组分的扩散系数，进而间接获得分子量。
In this paper, several new developments aimed at system optimization are reported for flow field-flow fractionation (FFF). These developments Include the application of field programming to flow FFF, the design and application of a new outlet stream splitter, and the realization of separation using flow rates roughly an order-of-magnitude higher than those previously employed In flow FFF. It is shown how each of these developments aids In the optimization of overall performance as measured by resolution, speed, sample detectability, and avoidance of overloading. Experimental results with sulfonated polystyrene samples are presented to Illustrate the new developments. The associated theoretical back-ground is provided.Among the family of macromolecular fractionation/char-acterization methods termed field-flow fractionation (FFF)(1-5), flow FFF is probably the most widely applicable. It requires only that the sample be soluble or dispersible in some carrier liquid and that a semipermeable membrane can be found which will retain the sample. Separation is based on differences in diffusion coefficient, for which the relationship to molecular weight is often well characterized. With the help of FFF theory, it is possible to directly obtainthe diffusion coefficients of components, and, by implication, the molecular weights from the elution profile of a complex sample following separation.
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影响因子：
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作者：
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DOI：
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发表时间：
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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