The Influence of Infection by Different Leishmania (Viannia) braziliensis Isolates on the Pathogenesis of Disseminated Leishmaniasis.
The Influence of Infection by Different Leishmania (Viannia) braziliensis Isolates on the Pathogenesis of Disseminated Leishmaniasis.
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DOI：
10.3389/fcimb.2021.740278
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发表时间：
2021
影响因子：
5.7
通讯作者：
Carvalho EM
中科院分区：
文献类型：
--
作者：
Oliveira WN;Dórea AS;Carneiro PP;Nascimento MT;Carvalho LP;Machado PRL;Schriefer A;Bacellar O;Carvalho EM
播散性利什曼病（DL）是巴西利什曼原虫（Viannia）感染的一种新出现的严重形式，其定义为存在10个和多达1，000个皮肤病变。寄生虫传播的潜在机制仍不清楚。不同种间的基因型差异。braziliensis与不同临床形式的疾病有关。本研究比较了从皮肤利什曼病（CL）和DL患者中获得的单核细胞对L。这两种临床形式的疾病的巴西分离物。从DL和CL患者中获得的单核细胞用不同的L.巴西分离株的感染细胞数、寄生虫负荷、呼吸爆发、TLR2和TLR4表达和细胞因子产生。与来自CL患者的分离株相比，DL分离株感染更多的单核细胞，诱导更大的呼吸爆发和更多的细胞因子产生，而不管单核细胞的来源（DL或CL）。然而，更大的寄生虫繁殖和更高的TLR2和TLR4的表达被认为是在单核细胞从DL患者相比，CL感染后DL分离株。我们的研究结果表明寄生虫基因型和宿主因素参与DL的发病机制。
Disseminated Leishmaniasis (DL) is an emerging and severe form of Leishmania (Viannia) braziliensis infection defined by the presence of 10 and up to more than 1,000 skin lesions. The mechanisms underlying parasite dissemination remain unknown. Genotypic differences among species of L. braziliensis have been associated with different clinical forms of disease. The present work compared the function of monocytes obtained from patients with cutaneous leishmaniasis (CL) and DL in response to infection with L. braziliensis isolates of both these two clinical forms of disease. Mononuclear cells obtained from DL and CL patients were infected with different L. braziliensis isolates, and numbers of infected cells, parasite load, respiratory burst, TLR2 and TLR4 expression and cytokine production were evaluated. DL isolates infected more monocytes, induced greater respiratory burst, and more cytokine production compared to isolates from CL patients regardless of the origin of monocytes (DL or CL). However, greater parasite multiplication and higher TLR2 and TLR4 expression were seen in monocytes from DL patients compared to CL following infection with DL isolates. Our results indicate the participation of both parasite genotype and host factors in the pathogenesis of DL.
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影响因子：
3.7
作者：
Leopoldo PT;Machado PR;Almeida RP;Schriefer A;Giudice A;de Jesus AR;Ho JL;Guimarães LH;Bacellar O;Carvalho EM
通讯作者：
Carvalho EM
影响因子：
11.2
作者：
Harlin H;Meng Y;Peterson AC;Zha Y;Tretiakova M;Slingluff C;McKee M;Gajewski TF
通讯作者：
Gajewski TF
影响因子：
4.6
作者：
Lonardoni MV;Barbieri CL;Russo M;Jancar S
通讯作者：
Jancar S
影响因子：
4.1
作者：
Díaz, NL;Fernández, M;Tapia, FJ
通讯作者：
Tapia, FJ
影响因子：
6.4
作者：
Khouri, Ricardo;Santos, Gilvaneia Silva;Van Weyenbergh, Johan
通讯作者：
Van Weyenbergh, Johan