Horizontal gene transfer in an acid mine drainage microbial community.
Horizontal gene transfer in an acid mine drainage microbial community.
复制标题
酸性矿山排水微生物群落的水平基因转移
DOI：
10.1186/s12864-015-1720-0
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发表时间：
2015-07-04
期刊：
影响因子：
4.4
通讯作者：
Zhu H
中科院分区：
文献类型：
--
作者：
Guo J;Wang Q;Wang X;Wang F;Yao J;Zhu H
背景水平基因转移（Horizontal gene transfer，HGT）广泛存在于原核生物的基因组中.然而，HGT在微生物群落成员之间和进化中的作用仍然很大程度上未知。随着宏基因组学的出现，研究来自自然环境的微生物群落中成员之间的遗传物质的水平流动是不平凡的。由于缺乏合适的宏基因组学基因转移检测方法，本研究以一个低复杂度的酸性矿山废水（AMD）中基因组接近完整的微生物为研究对象，对可能的基因转移事件进行检测，并提出其生物学意义。我们预测了119个可能的转移基因。其中，14个HGT事件被确定为AMD成员之间的转移事件。对14个转基因的进一步分析表明，HGT事件影响了AMD中古菌或细菌的功能进化，并可能塑造了AMD中古菌的群落结构，如G-plasma通过HGT在AMD中的优势地位。HGT和群落进化之间的相互联系对于理解微生物群落的形成和发展至关重要。
BackgroundHorizontal gene transfer (HGT) has been widely identified in complete prokaryotic genomes. However, the roles of HGT among members of a microbial community and in evolution remain largely unknown. With the emergence of metagenomics, it is nontrivial to investigate such horizontal flow of genetic materials among members in a microbial community from the natural environment. Because of the lack of suitable methods for metagenomics gene transfer detection, microorganisms from a low-complexity community acid mine drainage (AMD) with near-complete genomes were used to detect possible gene transfer events and suggest the biological significance.ResultsUsing the annotation of coding regions by the current tools, a phylogenetic approach, and an approximately unbiased test, we found that HGTs in AMD organisms are not rare, and we predicted 119 putative transferred genes. Among them, 14 HGT events were determined to be transfer events among the AMD members. Further analysis of the 14 transferred genes revealed that the HGT events affected the functional evolution of archaea or bacteria in AMD, and it probably shaped the community structure, such as the dominance of G-plasma in archaea in AMD through HGT.ConclusionsOur study provides a novel insight into HGT events among microorganisms in natural communities. The interconnectedness between HGT and community evolution is essential to understand microbial community formation and development.
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影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
3.6
作者：
Cotter, PD;Gahan, CGM;Hill, C
通讯作者：
Hill, C
影响因子：
56.9
作者：
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通讯作者：
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影响因子：
3.9
作者：
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通讯作者：
Rosato, Antonio
影响因子：
10.7
作者：
Filee, Jonathan;Bapteste, Eric;Krisch, H. M.
通讯作者：
Krisch, H. M.