Co-increase of Nerve Fibers and HLA-DRand/or Factor-XIIIa-expressing Dendritic Cells in Dentinal Caries-affected Regions of the Human Dental Pulp: An Immunohistochemical Study
Co-increase of Nerve Fibers and HLA-DRand/or Factor-XIIIa-expressing Dendritic Cells in Dentinal Caries-affected Regions of the Human Dental Pulp: An Immunohistochemical Study
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人牙髓受龋齿影响区域神经纤维和表达 HLA-DR 和/或因子 XIIIa 的树突状细胞共同增加：一项免疫组织化学研究
DOI：
10.1177/00220345990780100401
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发表时间：
1999
影响因子：
7.6
通讯作者：
H. Suda
中科院分区：
文献类型：
--
作者：
K. Sakurai;T. Okiji;H. Suda
神经-免疫相互作用在牙本质/牙髓复合体的免疫防御中起一定的调节作用。在这项研究中，我们进行了同步免疫组织化学观察神经元素和牙髓树突状细胞（PDCs）在人类龋牙，获得神经免疫相互作用的形态学证据牙本质小管源性龋刺激。研究了具有无牙髓暴露龋齿病变的人类第三磨牙。用抗HLA-DR、抗凝血因子XIIIa和抗CD 14作为PDC标记物，用抗低亲和力神经生长因子受体（NGFR）、抗蛋白基因产物9.5和抗降钙素基因相关肽作为神经标记物进行免疫过氧化物酶染色。龋牙通常表现出局部积累的PDCs和神经纤维免疫反应的每一个标记，在para-odontoblastic区域对应于牙髓端龋牙本质小管。半定量数字光密度测定显示，对应于因子-XIIIa-和NGFR免疫反应性的像素数显着高于在非龋区的相同的牙齿，以及在相应的区域的完整的牙齿的龋区。根据龋深对样本进行分类，结果表明，表浅龋牙齿的共同增加最为明显。修复性牙本质龋损牙中PDCs的增加不明显。这些结果表明，牙髓神经和PDCs的反应迅速，积极的牙本质小管源性龋刺激。这两种结构的同步积累表明神经免疫相互作用的机会增加，这可能在牙髓病理过程的调节中具有重要意义。
Neuro-immune interaction has been suggested to play some modulatory role in the immunodefense of the dentin/pulp complex. In this study, we performed a simultaneous immunohistochemical observation of neural elements and pulpal dendritic cells (PDCs) on human carious teeth, to obtain morphological evidence for neuro-immune interaction in response to dentinal tubule-derived carious stimuli. Human third molars bearing a pulp-exposure-free caries lesion were studied. Immunoperoxidase staining was performed with anti-HLA-DR, anti-coagulation factor XIIIa, and anti-CD14 as PDC markers, and anti-low-affinity nerve growth factor receptor (NGFR), anti-protein gene products 9.5, and anti-calcitonin gene-related peptide as nerve markers. The carious teeth usually exhibited localized accumulation of both PDCs and nerve fibers immunoreactive to each marker, in the para-odontoblastic region corresponding to the pulpal end of carious dentinal tubules. Semi-quantitative digital densitometry revealed that pixel numbers corresponding to factor-XIIIa- and NGFRimmunoreactivity were significantly higher in the carious regions than those in the non-carious regions of the same teeth as well as those in the corresponding regions of intact teeth. Classification of specimens with respect to caries depth showed that the co-increase was most apparent in teeth with superficial caries. The increase of PDCs was less pronounced in carious teeth with reparative dentin. These findings suggest that both pulpal nerves and PDCs respond promptly and actively to dentinal tubule-derived carious stimuli. The synchronized accumulation of the two structures suggests an increased opportunity for neuro-immune interaction that may be of significance in the modulation of pathological processes in the dental pulp.
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影响因子：
4.4
作者：
Y. Nong;R. Titus;J. Ribeiro;H. Remold
通讯作者：
Y. Nong;R. Titus;J. Ribeiro;H. Remold
影响因子：
4.4
作者：
D. Payan;D. Brewster;E. Goetzl
通讯作者：
D. Payan;D. Brewster;E. Goetzl
神经生长因子在有髓鞘伤害感受器发育中的作用。
DOI：
10.1523/jneurosci.12-05-01896.1992
发表时间：
1992
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Lewin,GR;Ritter,AM;Mendell,LM
通讯作者：
Mendell,LM
影响因子：
56.9
作者：
M. Lotz;J. Vaughan;D. Carson
通讯作者：
M. Lotz;J. Vaughan;D. Carson
影响因子：
3
作者：
BYERS, MR
通讯作者：
BYERS, MR