Mutagenicity of the nuclease activity of 1,10-phenanthroline-copper ion.

Mutagenicity of the nuclease activity of 1,10-phenanthroline-copper ion.
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1,10-菲咯啉-铜离子的核酸酶活性的致突变性。

DOI:
10.1016/s0006-291x(88)81090-8
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发表时间:
1988
影响因子:
3.1
通讯作者:
Sigman,DS
Sigman,DS
中科院分区:
生物学4区
文献类型:
--
作者:
Feig,AL;Thederahn,T;Sigman,DS

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The nuclease activity of 1,10-phenanthroline-copper functions intracellularly. This was shown by its mutagenicity in the Ames Test using the tester strain TA102 and the in vivo nicking of plasmids derived from this strain. In vivo DNA strand scission requires all the components essential for the in vitro activity: 1,10-phenanthroline, cupric ion, thiol and hydrogen peroxide. Although60Co gamma radiation potentiates the nuclease activity of 1,10-phenanthroline-copper ion in vitro via a superoxide dependent pathway, it does not promote significant mutagenesis in vivo at exposure levels below cytotoxicity.
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