Chromatin accessibility-based characterisation of brain gene regulatory networks in three distinct honey bee polyphenisms.
Chromatin accessibility-based characterisation of brain gene regulatory networks in three distinct honey bee polyphenisms.
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DOI：
10.1093/nar/gkac992
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发表时间：
2022-11-11
影响因子：
14.9
通讯作者：
Hurd, Paul J.
中科院分区：
文献类型：
--
作者：
Lowe, Robert;Wojciechowski, Marek;Ellis, Nancy;Hurd, Paul J.
蜜蜂基因组有能力产生三种表型不同的生物体（两种二倍体雌性种姓：蜂王和工蜂，以及一种单倍体雄性雄蜂）。以往的研究表明，代谢通量通过表观遗传调控作用于蜜蜂营养驱动的表型可塑性。然而，建立组织和多型特异性表观基因组和基因表达程序的顺式作用DNA调控元件仍不清楚。使用高分辨率多组学方法，包括通过测序测定转座酶可接近的染色质（ATAC-seq）、RNA-seq和ChIP-seq，我们产生了所有三种成年蜜蜂表型的调控景观的第一个全基因组图谱，鉴定了蜂王中> 5000个调控区域，工蜂中7500个调控区域和雄蜂中6500个调控区域，其中绝大多数这些位点位于内含子区域内。这些区域由H3 K27 ac的正富集和H3 K4 me 3的耗竭限定，并且显示与基因表达的正相关。使用ATAC-seq足迹法，我们确定了蜂王、工蜂和雄蜂特异性转录因子的占有率，并揭示了新的表型特异性调控网络，该网络鉴定了先前在蜜蜂中涉及种姓决定和成年行为成熟的两种关键核受体;蜕皮激素受体和超气门。总的来说，这项研究提供了新的见解关键基因调控网络，这些网络与蜜蜂中这些不同的多型现象。
The honey bee genome has the capacity to produce three phenotypically distinct organisms (two diploid female castes: queen and worker, and a haploid male drone). Previous studies have implicated metabolic flux acting via epigenetic regulation in directing nutrition-driven phenotypic plasticity in the honey bee. However, the cis-acting DNA regulatory elements that establish tissue and polyphenism -specific epigenomes and gene expression programmes, remain unclear. Using a high resolution multiomic approach including assay for transposase-accessible chromatin by sequencing (ATAC-seq), RNA-seq and ChIP-seq, we produce the first genome-wide maps of the regulatory landscape across all three adult honey bee phenotypes identifying > 5000 regulatory regions in queen, 7500 in worker and 6500 in drone, with the vast majority of these sites located within intronic regions. These regions are defined by positive enrichment of H3K27ac and depletion of H3K4me3 and show a positive correlation with gene expression. Using ATAC-seq footprinting we determine queen, worker and drone -specific transcription factor occupancy and uncover novel phenotype-specific regulatory networks identifying two key nuclear receptors that have previously been implicated in caste-determination and adult behavioural maturation in honey bees; ecdysone receptor and ultraspiracle. Collectively, this study provides novel insights into key gene regulatory networks that are associated with these distinct polyphenisms in the honey bee.
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高度社会性蜜蜂意大利蜜蜂种姓分化的分子决定因素。
影响因子：
--
作者：
Barchuk AR;Cristino AS;Kucharski R;Costa LF;Simões ZL;Maleszka R
通讯作者：
Maleszka R
影响因子：
4.5
作者：
Ament SA;Wang Y;Chen CC;Blatti CA;Hong F;Liang ZS;Negre N;White KP;Rodriguez-Zas SL;Mizzen CA;Sinha S;Zhong S;Robinson GE
通讯作者：
Robinson GE
影响因子：
2.2
作者：
Barchuk, Angel Roberto;Figueiredo, Vera Lucia C.;Simoes, Zila L. P.
通讯作者：
Simoes, Zila L. P.
影响因子：
7.7
作者：
Jones BM;Rao VD;Gernat T;Jagla T;Cash-Ahmed AC;Rubin BE;Comi TJ;Bhogale S;Husain SS;Blatti C;Middendorf M;Sinha S;Chandrasekaran S;Robinson GE
通讯作者：
Robinson GE
影响因子：
10.5
作者：
HIGASHIJIMA, SI;KOJIMA, T;SAIGO, K
通讯作者：
SAIGO, K