Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro.
Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro.
复制标题
Wnt5a介导补肾活血汤对骨髓间充质干细胞体外迁移的影响
DOI：
10.1186/s13020-018-0200-2
复制
发表时间：
2018
期刊：
影响因子：
4.9
通讯作者：
Luo Y
中科院分区：
文献类型：
--
作者：
Shen W;Luo H;Xu L;Wu Z;Chen H;Liu Y;Yu L;Hu L;Wang B;Luo Y
补肾活血方对骨折愈合有显著作用，但其作用机制尚不清楚。本研究旨在探讨补阳还五汤是否通过Wnt 5a信号通路促进骨髓间充质干细胞（BMSC）迁移。用石油醚提取BHD，并分析其组成。使用细胞计数试剂盒-8测定法测量在各种浓度的BHD存在下24、48和72小时的细胞活力。采用Transwell实验和伤口愈合实验观察BMSCs的迁移能力。慢病毒载体用于敲低Wnt 5a。聚合酶链反应和蛋白质印迹分析用于进一步比较组间Wnt 5a信号传导组分在mRNA和蛋白质水平上的差异。BHD处理组显示出增加的迁移能力和Wnt 5a表达。使用慢病毒敲低Wnt 5a显著抑制BHD的作用，这意味着BHD通过激活Wnt 5a促进BMSC迁移能力。BHD可以增强BMSC迁移，可能是通过激活Wnt 5a信号传导。本文的在线版本（10.1186/s13020-018-0200-2）包含补充材料，可供授权用户使用。
Bushen Huoxue decoction (BHD) has a significant effect on fracture rehabilitation, yet its underlying mechanism is still unknown. The purpose of this study was to explore whether BHD promotes bone marrow mesenchymal stem cell (BMSC) migration through the Wnt5a signalling pathway. BHD was extracted by petroleum, and its composition was analysed. Cell viability in the presence of various concentrations of BHD for 24, 48 and 72 h was measured using a Cell Counting Kit-8 assay. Transwell assays and wound healing assays were used to observe the migration ability of BMSCs. Lentiviral vectors were used to knock down Wnt5a. Polymerase chain reaction and Western blot analyses were used to further compare Wnt5a signalling components at the mRNA and protein levels between groups. BHD treatment groups showed increased migration ability and Wnt5a expression. Knocking down Wnt5a using a lentivirus significantly inhibited the effects of BHD, which implies that BHD promotes BMSC migration ability through activation of Wnt5a. BHD can enhance BMSC migration, possibly by activating Wnt5a signalling. The online version of this article (10.1186/s13020-018-0200-2) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
2.7
作者：
Zhu N;Qin L;Luo Z;Guo Q;Yang L;Liao D
通讯作者：
Liao D
影响因子：
2.7
作者：
Slusarski, DC;YangSnyder, J;Moon, RT
通讯作者：
Moon, RT
影响因子：
7.9
作者：
Lim, Sun-Hye;Ha, Tae-Youl;Kim, Suna
通讯作者：
Kim, Suna
影响因子：
2.6
作者：
Ferguson, C;Alpern, E;Helms, JA
通讯作者：
Helms, JA
影响因子：
2.9
作者：
Liu Wei;Wu Yuan-hao;Yang Kuo
通讯作者：
Yang Kuo