Characterization of expressed human meibum using hyperspectral stimulated Raman scattering microscopy.
Characterization of expressed human meibum using hyperspectral stimulated Raman scattering microscopy.
复制标题
DOI：
10.1016/j.jtos.2018.10.003
复制
发表时间：
2019-01
期刊：
影响因子：
--
通讯作者：
Jester JV
中科院分区：
文献类型：
--
作者：
Paugh JR;Alfonso-Garcia A;Nguyen AL;Suhalim JL;Farid M;Garg S;Tao J;Brown DJ;Potma EO;Jester JV
本研究检验了高光谱受激拉曼散射 (hsSRS) 显微镜是否可以检测正常和患有睑板腺功能障碍的蒸发性干眼受试者睑脂与蛋白质组成的差异。对受试者的泪液破裂时间（TBUT）、染色、睑脂表达和腺体脱落进行评估。使用 CH 拉伸区域 (2800 – 3050 cm−1) 中的 SRS 振动特征分析表达的睑脂。使用顶点成分分析和 K 均值聚类将光谱特征分为包含高脂质 (G1) 到高蛋白质 (G4) 的四个部分。可以使用合并睑脂对 33 名受试者进行统计分析（13 名受试者泪膜稳定（TBUT > 10 秒），20 名受试者泪膜不稳定（TBUT ≤ 10 秒）。在 G1 分数（中位数分别为 0.164 和 0.020；p = 0.012）和 G2 分数（中位数）方面，发现不稳定 TBUT 受试者与稳定 TBUT 受试者的睑脂存在显着差异。分别为 0.244 和 0.272；G3 和 G4 分数与另一眼的合并样本没有显着差异，而眼睑部分样本（鼻、中央和颞部）G2:G3 分数成分没有显着差异 (p = 0.449)。 −0.351；p = 0.045）。hsSRS 显微镜可以对人类表达的睑脂进行成分分析，这些结果与 TBUT 的变化相关。这些结果支持了 hsSRS 可能有助于对睑脂质量进行分类和评估治疗效果的假设。
This study examined whether hyperspectral stimulated Raman scattering (hsSRS) microscopy can detect differences in meibum lipid to protein composition of normal and evaporative dry eye subjects with meibomian gland dysfunction. Subjects were evaluated for tear breakup time (TBUT), staining, meibum expression and gland dropout. Expressed meibum was analyzed using SRS vibrational signatures in the CH stretching region (2800 – 3050 cm−1). Vertex component analysis and K-means clustering were used to group the spectral signatures into four fractions containing high lipid (G1) to high protein (G4). Thirty-three subjects could be statistically analyzed using pooled meibum (13 with stable tear films (TBUTs > 10 seconds) and 20 with unstable tear films (TBUTs ≤ 10 seconds). Significant differences in meibum from subjects with unstable vs. stable TBUTs were found for the G1 fraction (medians 0.164 and 0.020, respectively; p = 0.012) and the G2 fraction (medians 0.244 and 0.272, respectively; p = 0.045). No differences were observed for the G3 and G4 fractions. Single orifice samples were not significantly different vs. pooled samples from the fellow eye, and eyelid sector samples (nasal, central and temporal) G2:G3 fractional components were not significantly different (p = 0.449). Spearman analysis suggested a significant inverse correlation between G1 fraction and TBUT (R = −0.351; p = 0.045). hsSRS microscopy allows compositional analysis of expressed meibum from humans which correlated to changes in TBUT. These findings support the hypothesis that hsSRS may be useful in classifying meibum quality and evaluating the effects of therapy.
登录
查看更多内容
影响因子：
3.4
作者：
Borchman, Douglas;Yappert, Marta C.;Foulks, Gary N.
通讯作者：
Foulks, Gary N.
影响因子：
--
作者：
Nien, Chyong Jy;Massei, Salina;Lin, Gloria;Nabavi, Cameron;Tao, Jeremiah;Brown, Donald J.;Paugh, Jerry R.;Jester, James V.
通讯作者：
Jester, James V.
影响因子：
2.8
作者：
Pflugfelder, SC;Tseng, SCG;Reis, BL
通讯作者：
Reis, BL
影响因子：
13.7
作者：
Arita, Reiko;Itoh, Kouzo;Amano, Shiro
通讯作者：
Amano, Shiro
影响因子：
--
作者：
SHIMAZAKI, J;SAKATA, M;TSUBOTA, K
通讯作者：
TSUBOTA, K