Effects of age and dysfunction on human meibomian glands.
Effects of age and dysfunction on human meibomian glands.
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DOI：
10.1001/archophthalmol.2011.69
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发表时间：
2011-04
影响因子：
--
通讯作者：
Jester, James V.
中科院分区：
文献类型：
--
作者：
Nien, Chyong Jy;Massei, Salina;Lin, Gloria;Nabavi, Cameron;Tao, Jeremiah;Brown, Donald J.;Paugh, Jerry R.;Jester, James V.
确定可能与睑板腺功能障碍（MGD）相关的人睑板腺年龄相关变化。使用了36名接受眦成形术的患者（年龄范围，18-95岁，19名女性，17名男性）的多余眼睑组织。记录皮肤病史、年龄和是否存在MGD。将样本冷冻、切片，并用过氧化物酶体增殖物激活受体γ（PPARγ）的特异性抗体染色，以识别睑板细胞分化，用Ki 67核抗原染色，以识别循环细胞，用CD 45染色，以识别炎性细胞浸润。在2名最年轻的受试者（18岁和44岁）中，PPARγ染色显示细胞质和细胞核定位。老年人（>60岁）主要表现为核染色，31例受试者中有17例的细胞质染色仅限于基底腺泡细胞。基于线性回归分析，与老年受试者相比，年轻受试者中Ki 67阳性染色基底细胞的数量显著升高（r2= 0.35; P <.001）。MG表达分级与CD 45细胞浸润之间也存在显著相关性（r =0.414; P = 0.05）。这些结果表明，老化的人睑板腺显示出与MGD的发展相关的降低的睑板细胞分化和细胞周期。研究结果还表明，改变的PPARγ信号可能导致腺泡萎缩和年龄相关的分泌不足MGD的发展。睑板腺功能障碍和蒸发性干眼是常见的年龄相关性眼睑疾病。了解MGD的潜在机制可能会导致开发新的治疗策略来治疗这种疾病。
To identify age-related changes in human meibomian glands that may be associated with meibomian gland dysfunction (MGD). Excess eyelid tissue from 36 patients (age range, 18–95 years, 19 female, 17 male) who underwent canthoplasty procedures were used. Dermatologic history, age, and presence of MGD were recorded. Samples were frozen, sectioned, and stained with specific antibodies against peroxisome proliferator–activated receptor γ(PPARγ) to identify meibocyte differentiation, Ki67 nuclear antigen to identify cycling cells, and CD45 to identify inflammatory cell infiltration. Staining for PPARγ showed cytoplasmic and nuclear localization in the 2 youngest subjects (ages, 18 and 44 years). Older individuals (>60 years) showed predominantly nuclear staining, with cytoplasmic staining limited to the basal acinar cells in 17 of 31 subjects. The number of Ki67 positively stained basal cells were significantly elevated in the younger compared with older subjects based on linear regression analysis (r2= 0.35; P <.001). There was also a significant correlation between MG expression grade and CD45 cell infiltration (r =0.414; P =.05). These results indicate that aging human meibomian glands show decreased meibocyte differentiation and cell cycling that is associated with the development of MGD. Findings also suggest that altered PPARγ signaling may lead to acinar atrophy and development of an age-related hyposecretory MGD. Meibomian gland dysfunction and evaporative dry eye are common age-related eyelid disorders. Understanding the underlying mechanism of MGD may lead to the development of novel therapeutic strategies to treat this disease.
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DOI：
10.1016/j.plefa.2005.04.008
发表时间：
2005-07-01
影响因子：
3
作者：
Hammarstedt, A;Andersson, CX;Smith, U
通讯作者：
Smith, U
影响因子：
3.2
作者：
Guillon, Michel;Maissa, Cecile
通讯作者：
Maissa, Cecile
影响因子：
10.3
作者：
Makrantonaki, E.;Zouboulis, C. C.
通讯作者：
Zouboulis, C. C.
影响因子：
2.8
作者：
HYKIN, PG;BRON, AJ
通讯作者：
BRON, AJ
影响因子：
2.8
作者：
MATHERS, WD;SHIELDS, WJ;JESTER, JV
通讯作者：
JESTER, JV