Changes in human meibum lipid with meibomian gland dysfunction using principal component analysis.
Changes in human meibum lipid with meibomian gland dysfunction using principal component analysis.
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DOI：
10.1016/j.exer.2010.05.014
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发表时间：
2010-08
影响因子：
3.4
通讯作者：
Foulks, Gary N.
中科院分区：
文献类型：
--
作者：
Borchman, Douglas;Yappert, Marta C.;Foulks, Gary N.
人类代谢脂的相变温度和构象随年龄和代谢腺功能障碍的变化已经被量化，但分析的红外光谱范围不到1%。其余99%的光谱范围用主成分分析（PCA）进行了分析，证实了我们之前的研究，并进一步揭示了代谢随年龄和疾病发生的变化。对41例诊断为睑板腺功能障碍（Md）的患者和32例正常供体（Mn）的睑板进行了红外光谱测定。采用主成分分析（PCA）量化各光谱间的差异，并利用红外羰基和酰胺I和II波段定量代谢蛋白。一个光谱训练集被用来区分Mn和Md，准确率为93%。这表明，特定的光谱区域（特征向量）包含有关组件（变量），睑板腺功能障碍的组成和结构信息。主特征向量的光谱特征表明，与Mn相比，Md含有更多的蛋白质，相对较少的CH3和cis=CH波段强度。通过相对红外波段强度确定了蛋白质的含量。我们的研究支持这样一种观点，即组成差异导致睑板腺功能障碍导致睑板较少流动性和粘性，从而减少临床观察到的从睑板腺孔流出的脂质。该研究还证明了红外光谱和PCA结合作为区分Mn和Md的诊断工具的能力。
Changes in the phase transition temperatures and conformation of human meibum lipid with age and meibomian gland dysfunction have been quantified but with analysis of less than 1% of the infrared spectral range. The remaining 99 % of the spectral range was analyzed with principal component analysis (PCA) and confirms our previous studies and reveal further insights into changes that occur in meibum with age and disease. Infrared spectra of meibum from 41 patients diagnosed with meibomian gland dysfunction (Md) and 32 normal donors (Mn) were measured. Principal component analysis (PCA) was used to quantify the variance among the spectra and meibum protein was quantified using the infrared carbonyl and amide I and II bands. A training set of spectra was used to discriminate between Mn and Md with an accuracy of 93 %. This shows that certain spectral regions (eigenvectors) contain compositional and structural information about the component (variable), meibomian gland dysfunction. The spectral features of the major eigenvector indicate that Md contains more protein and relatively less CH3 and cis=CH band intensity compared to Mn. The amount of protein was confirmed from relative infrared band intensities. Our study supports the idea that compositional differences result in meibum that is less fluid and more viscous with meibomian gland dysfunction so that less lipid flows out of the meibomian gland orifice as observed clinically. This study also demonstrates the power of the combination of infrared spectroscopy and PCA as a diagnostic tool that discriminates between Mn and Md.
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