Comparison of Antibacterial and Immunological Properties of Mesenchymal Stem/Stromal Cells from Equine Bone Marrow, Endometrium, and Adipose Tissue.
Comparison of Antibacterial and Immunological Properties of Mesenchymal Stem/Stromal Cells from Equine Bone Marrow, Endometrium, and Adipose Tissue.
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DOI：
10.1089/scd.2017.0241
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发表时间：
2018-11-01
影响因子：
4
通讯作者：
Esteves CL
中科院分区：
文献类型：
--
作者：
Cortés-Araya Y;Amilon K;Rink BE;Black G;Lisowski Z;Donadeu FX;Esteves CL
马间充质干/基质细胞（MSCs）是广泛用于治疗肌肉骨骼损伤的多能细胞，并且将其应用扩展到非骨科条件具有重大意义。间充质干细胞具有抗菌和免疫调节特性，可能与对抗感染有关;然而，使用不同来源的间充质干细胞的比较研究尚未在马中进行，这是本研究的重点。我们的研究结果表明，MSC条件培养基衰减大肠杆菌的生长，这种效果是，平均而言，子宫内膜（EM）衍生和脂肪组织（AT）衍生的MSC比骨髓（BM）衍生的MSC更明显。此外，抗菌脂笼蛋白-2在EM-MSC中的表达水平平均高于AT-MSC和BM-MSC，细菌组分脂多糖（LPS）刺激所有三种MSC类型的生产。我们还发现，MSC表达白细胞介素-6（IL-6），IL-8，单核细胞趋化蛋白-1，趋化因子配体-5，和Toll样受体4，并且，一般来说，这些细胞因子在所有细胞类型中被LPS诱导。巨噬细胞标记物集落刺激因子1受体在BM-MSCs和EM-MSCs中检测到低表达水平，但在AT-MSCs中未检测到。总之，这些发现表明，来自EM，AT和BM的马MSC具有直接和间接的抗菌特性，这些特性在来自不同来源的MSC之间可能有所不同，并且可以用于改善马的再生疗法。
Equine mesenchymal stem/stromal cells (MSCs) are multipotent cells that are widely used for treatment of musculoskeletal injuries, and there is significant interest in expanding their application to nonorthopedic conditions. MSCs possess antibacterial and immunomodulatory properties that may be relevant for combating infection; however, comparative studies using MSCs from different origins have not been carried out in the horse, and this was the focus of this study. Our results showed that MSC-conditioned media attenuated the growth of Escherichia coli, and that this effect was, on average, more pronounced for endometrium (EM)-derived and adipose tissue (AT)-derived MSCs than for bone marrow (BM)-derived MSCs. In addition, the antimicrobial lipocalin-2 was expressed at mean higher levels in EM-MSCs than in AT-MSCs and BM-MSCs, and the bacterial component lipopolysaccharide (LPS) stimulated its production by all three MSC types. We also showed that MSCs express interleukin-6 (IL-6), IL-8, monocyte chemoattractant protein-1, chemokine ligand-5, and Toll-like receptor 4, and that, in general, these cytokines were induced in all cell types by LPS. Low expression levels of the macrophage marker colony-stimulating factor 1 receptor were detected in BM-MSCs and EM-MSCs but not in AT-MSCs. Altogether, these findings suggest that equine MSCs from EM, AT, and BM have both direct and indirect antimicrobial properties that may vary between MSCs from different origins and could be exploited toward improvement of regenerative therapies for horses.
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影响因子：
5.2
作者：
Hall, Sean R. R.;Tsoyi, Konstantin;Ith, Bonna;Padera, Robert F., Jr.;Lederer, James A.;Wang, Zhihong;Liu, Xiaoli;Perrella, Mark A.
通讯作者：
Perrella, Mark A.
影响因子：
1.8
作者：
Carvalho, Armando de Mattos;Garcia Alves, Ana Liz;Deffune, Elenice
通讯作者：
Deffune, Elenice
影响因子：
1.8
作者：
Karagianni, Anna E.;Kapetanovic, Ronan;McGorum, Bruce C.;Hume, David A.;Pirie, Scott R.
通讯作者：
Pirie, Scott R.
影响因子：
7.5
作者：
Harman RM;Yang S;He MK;Van de Walle GR
通讯作者：
Van de Walle GR
影响因子：
3.7
作者：
Brandau S;Jakob M;Bruderek K;Bootz F;Giebel B;Radtke S;Mauel K;Jäger M;Flohé SB;Lang S
通讯作者：
Lang S