Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.
Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.
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DOI：
10.1371/journal.pone.0106903
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lang S
中科院分区：
文献类型：
--
作者：
Brandau S;Jakob M;Bruderek K;Bootz F;Giebel B;Radtke S;Mauel K;Jäger M;Flohé SB;Lang S
间充质干细胞（MSC）参与炎症和先天免疫的调节，例如通过响应病原体来源的信号和通过调节先天免疫细胞的功能。骨髓间充质干细胞和外周组织间充质干细胞具有共同的基本细胞生物学功能。然而，目前尚不清楚这些MSC是否对微生物挑战表现出不同的反应，以及这种反应是否随后通过MSC调节炎症细胞。我们从人骨髓（bmMSCs）和人唾液腺（pgMSCs）中分离出MSCs。通过流式细胞术和定量PCR测定TLR4和LPS应答分子的表达水平。通过ELISA测定细胞因子释放。在体外测试来自未刺激和LPS刺激的MSC的上清液对多形核中性粒细胞（PMN）的募集、细胞因子分泌、细菌清除和氧化爆发的影响。尽管存在微小的数量差异，但bmMSC和pgMSC对细菌内毒素表现出相似的细胞生物学反应。两种类型的MSC增强了PMN的抗微生物功能，特别是bmMSC，LPS刺激进一步增强了MSC介导的PMN活化。这项研究表明，骨髓间充质干细胞可能有助于感染和炎症的决议，通过促进中性粒细胞的抗微生物活性。这种性质是由骨髓和外周腺组织来源的MSC发挥。
Mesenchymal stem cells (MSCs) participate in the regulation of inflammation and innate immunity, for example by responding to pathogen-derived signals and by regulating the function of innate immune cells. MSCs from the bone-marrow and peripheral tissues share common basic cell-biological functions. However, it is unknown whether these MSCs exhibit different responses to microbial challenge and whether this response subsequently modulates the regulation of inflammatory cells by MSCs. We isolated MSCs from human bone-marrow (bmMSCs) and human salivary gland (pgMSCs). Expression levels of TLR4 and LPS-responsive molecules were determined by flow cytometry and quantitative PCR. Cytokine release was determined by ELISA. The effect of supernatants from unstimulated and LPS-stimulated MSCs on recruitment, cytokine secretion, bacterial clearance and oxidative burst of polymorphonuclear neutrophil granulocytes (PMN) was tested in vitro. Despite minor quantitative differences, bmMSCs and pgMSCs showed a similar cell biological response to bacterial endotoxin. Both types of MSCs augmented anti-microbial functions of PMNs LPS stimulation, particularly of bmMSCs, further augmented MSC-mediated activation of PMN. This study suggests that MSCs may contribute to the resolution of infection and inflammation by promoting the anti-microbial activity of PMNs. This property is exerted by MSCs derived from both the bone-marrow and peripheral glandular tissue.
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影响因子：
48
作者：
Connick, Peter;Kolappan, Madhan;Crawley, Charles;Webber, Daniel J.;Patani, Rickie;Michell, Andrew W.;Du, Ming-Qing;Luan, Shi-Lu;Altmann, Daniel R.;Thompson, Alan J.;Compston, Alastair;Scott, Michael A.;Miller, David H.;Chandran, Siddharthan
通讯作者：
Chandran, Siddharthan
影响因子：
4
作者：
Jakob, Mark;Hemeda, Hatim;Brandau, Sven
通讯作者：
Brandau, Sven
影响因子：
2.8
作者：
Hsu, Shu-Ching;Wang, Li-Tzu;Chen, Hsin-Wei
通讯作者：
Chen, Hsin-Wei
影响因子：
2.6
作者：
Boehrnsen, Florian;Rotter, Nicole;Kramer, Jan
通讯作者：
Kramer, Jan
影响因子：
7.5
作者：
Jackson WM;Nesti LJ;Tuan RS
通讯作者：
Tuan RS