Rapid and simple detection of foot-and-mouth disease virus: Evaluation of a cartridge-based molecular detection system for use in basic laboratories.
Rapid and simple detection of foot-and-mouth disease virus: Evaluation of a cartridge-based molecular detection system for use in basic laboratories.
复制标题
DOI：
10.1111/tbed.12744
复制
发表时间：
2018-04
影响因子：
4.3
通讯作者：
Fowler VL
中科院分区：
文献类型：
--
作者：
Goller KV;Dill V;Madi M;Martin P;Van der Stede Y;Vandenberge V;Haas B;Van Borm S;Koenen F;Kasanga CJ;Ndusilo N;Beer M;Liu L;Mioulet V;Armson B;King DP;Fowler VL
高度传染性的跨界动物疾病，如口蹄疫，是对农场动物生产力的主要威胁。为了限制暴发的影响并采取有效步骤及时控制和根除疾病，快速和可靠的诊断系统至关重要。验证性诊断分析通常由有经验的操作员在专门实验室进行，在疾病负担最重的发展中国家，获得这种能力的机会往往有限。分子技术的进步使现代和可靠的技术得以实施，以便在基础实验室或甚至在笔端进行快速和简单的病原体检测。在这里，我们报告了一项评估用于检测口蹄疫病毒(FMDV)的全自动盒式实时RT-PCR诊断系统(Enigma Minilab®)的研究。该模块化系统集成了核酸提取和下游实时RT-PCR(RRT-PCR)。用连续稀释培养的口蹄疫病毒测定了该方法的分析灵敏度，并使用选定的牛、猪和羊源口蹄疫病毒阳性和阴性临床样本对该方法的性能进行了评估。该分析的稳健性通过国际实验室间熟练程度测试和部署到非洲实验室进行了评估。结果表明，该系统使用方便，检测口蹄疫病毒具有较高的灵敏度和特异度，与实验室标准方法基本相当。这种基于试剂盒的自动化实时RT-PCR系统用于检测口蹄疫病毒，是一种可靠且易于使用的诊断工具，可用于即使在偏远地区也能对急性感染的动物进行早期和快速的疾病检测。这种类型的系统可以很容易地部署在非洲等流行地区的常规监测中，或者也可以在发达国家使用。
Highly contagious transboundary animal diseases such as foot‐and‐mouth disease (FMD) are major threats to the productivity of farm animals. To limit the impact of outbreaks and to take efficient steps towards a timely control and eradication of the disease, rapid and reliable diagnostic systems are of utmost importance. Confirmatory diagnostic assays are typically performed by experienced operators in specialized laboratories, and access to this capability is often limited in the developing countries with the highest disease burden. Advances in molecular technologies allow implementation of modern and reliable techniques for quick and simple pathogen detection either in basic laboratories or even at the pen‐side. Here, we report on a study to evaluate a fully automated cartridge‐based real‐time RT‐PCR diagnostic system (Enigma MiniLab®) for the detection of FMD virus (FMDV). The modular system integrates both nucleic acid extraction and downstream real‐time RT‐PCR (rRT‐PCR). The analytical sensitivity of this assay was determined using serially diluted culture grown FMDV, and the performance of the assay was evaluated using a selected range of FMDV positive and negative clinical samples of bovine, porcine and ovine origin. The robustness of the assay was evaluated in an international inter‐laboratory proficiency test and by deployment into an African laboratory. It was demonstrated that the system is easy to use and can detect FMDV with high sensitivity and specificity, roughly on par with standard laboratory methods. This cartridge‐based automated real‐time RT‐PCR system for the detection of FMDV represents a reliable and easy to use diagnostic tool for the early and rapid disease detection of acutely infected animals even in remote areas. This type of system could be easily deployed for routine surveillance within endemic regions such as Africa or could alternatively be used in the developed world.
登录
查看更多内容
影响因子：
4.3
作者：
Knight-Jones, T. J. D.;Robinson, L.;Vosloo, W.
通讯作者：
Vosloo, W.
DOI：
10.2460/javma.2002.220.1636
发表时间：
2002-06-01
期刊：
JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
影响因子：
--
作者：
Callahan, JD;Brown, F;Nelson, WM
通讯作者：
Nelson, WM
影响因子：
0.8
作者：
Pacheco, J. M.;Stenfeldt, C.;Arzt, J.
通讯作者：
Arzt, J.
影响因子：
8.8
作者：
Rolfe, K. J.;Parmar, S.;Curran, M. D.
通讯作者：
Curran, M. D.
影响因子：
2.6
作者：
Knight-Jones TJ;Rushton J
通讯作者：
Rushton J