Rice Flowering Locus T 1 plays an important role in heading date influencing yield traits in rice.
Rice Flowering Locus T 1 plays an important role in heading date influencing yield traits in rice.
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DOI：
10.1038/s41598-017-05302-3
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发表时间：
2017-07-07
影响因子：
4.6
通讯作者：
Zhuang JY
中科院分区：
文献类型：
--
作者：
Zhu YJ;Fan YY;Wang K;Huang DR;Liu WZ;Ying JZ;Zhuang JY
水稻成花基因在提高籽粒产量中的重要作用已被广泛认识，其中许多关键基因调控着成花素的产生，但对这两个成花素基因本身的研究却很少。本研究首先利用籼稻品种珍汕97和密阳46的水稻开花基因座T1（Rice Flowing Locus T1，RFT1）近等基因系（Near Isogenic Lines，NILs）分析了RFT1基因的多效性，然后将RFT1基因导入粳稻品种中花11中，研究了RFT1基因的多效性。RFT1等位基因1997可延迟抽穗，增加株高、粒重、粒数和产量，表明RFT1在水稻生长发育中起重要作用。本研究还验证了利用一种新的遗传资源--序贯剩余杂合子（SeqRHs）进行QTL精细定位的潜力。SeqRHs的鉴定、近等基因系的构建和QTL的精细定位采用分步法。杂合片段和候选QTL区域逐渐缩小。最终，QTL区域被限定为包含单个基因的1.7kb区域。
Important role of flowering genes in enhancing grain productivity in rice has become well recognized for a number of key genes regulating the florigen production, but little has been known for the two florigen genes themselves. In this study, pleiotropism of Rice Flowering Locus T 1 (RFT1), one of the two florigen genes in rice, was firstly evaluated using near isogenic lines (NILs) carrying RFT1 alleles from the indica rice cultivars Zhenshan 97 (ZS97) and Milyang 46, respectively, and then determined by transformation of the RFT1 ZS97 allele into a japonica rice variety, Zhonghua 11. The RFT1 ZS97 allele was shown to delay heading and increase plant height, grain weight, grain number and grain yield, indicating that RFT1 plays an important role in the growth and development of rice. This study has also validated the potential of using a new type of genetic resource, sequential residual heterozygotes (SeqRHs), for QTL fine-mapping. A step-by-step approach was employed for SeqRHs identification, NIL development and QTL fine-mapping. The heterozygous segments and candidate QTL regions were gradually narrowed down. Eventually, the QTL region was delimited to a 1.7 kb region containing a single gene.
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影响因子：
5.4
作者：
Ebana, Kaworu;Shibaya, Taeko;Wu, Jianzhong;Matsubara, Kazuki;Kanamori, Hiroyuki;Yamane, Hiroko;Yamanouchi, Utako;Mizubayashi, Tatsumi;Kono, Izumi;Shomura, Ayahiko;Ito, Sachie;Ando, Tsuyu;Hori, Kiyosumi;Matsumoto, Takashi;Yano, Masahiro
通讯作者：
Yano, Masahiro
影响因子：
5.4
作者：
Chen, X;Temnykh, S;McCouch, SR
通讯作者：
McCouch, SR
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影响因子：
1.9
作者：
Dai, Wei-Min;Zhang, Ke-Qin;Zhuang, Jie-Yun
通讯作者：
Zhuang, Jie-Yun
影响因子：
4.9
作者：
Endo-Higashi N;Izawa T
通讯作者：
Izawa T
qHd1 的精细定位，qHd1 是水稻 (Oryza sativa L.) 产量性状具有多效性的小抽穗日期 QTL。
影响因子：
5.4
作者：
Chen, Jun-Yu;Guo, Liang;Ma, Huan;Chen, Yu-Yu;Zhang, Hong-Wei;Ying, Jie-Zheng;Zhuang, Jie-Yun
通讯作者：
Zhuang, Jie-Yun