Concentration dependence of vitamin C in combinations with vitamin E and zeaxanthin on light-induced toxicity to retinal pigment epithelial cells.
Concentration dependence of vitamin C in combinations with vitamin E and zeaxanthin on light-induced toxicity to retinal pigment epithelial cells.
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DOI：
10.1111/j.1751-1097.2012.01228.x
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发表时间：
2012-11
影响因子：
3.3
通讯作者：
Różanowski B
中科院分区：
文献类型：
--
作者：
Różanowska M;Bakker L;Boulton ME;Różanowski B
本研究的目的是确定增加浓度的抗坏血酸单独和与α-生育酚和玉米黄质的组合对视网膜色素上皮的光毒性的影响。在存在和不存在抗氧化剂的情况下，将ARPE-19细胞暴露于虎红和可见光。毒性通过细胞还原活性测定进行定量。暴露于可见光和光敏剂20分钟使细胞活力降低至约42%。亲脂性抗氧化剂分别将α-生育酚、玉米黄质及其组合的活力提高至~ 70%、~61%和~75%。在0.35、0.7和1.4 mM抗坏血酸存在下暴露后，细胞活力分别为~ 70%、56%和5%。在不存在和存在抗坏血酸的情况下，45分钟暴露分别将细胞死亡增加至约74%和>95%。在抗坏血酸的存在下，玉米黄质没有显着影响光毒性。α-生育酚及其与玉米黄质的组合增强抗坏血酸的保护作用，但不能防止抗坏血酸介导的有害作用。总之，抗坏血酸盐发挥光保护作用的浓度和暴露时间范围很窄，超过此范围会导致抗坏血酸盐介导的光细胞毒性增加。维生素E及其与玉米黄质的组合可以增强抗坏血酸的保护作用，但不能改善其有害作用。
The purpose of this study was to determine the effects of increasing concentration of ascorbate alone and in combinations with α-tocopherol and zeaxanthin on phototoxicity to the retinal pigment epithelium. ARPE-19 cells were exposed to rose bengal and visible light in the presence and absence of antioxidants. Toxicity was quantified by an assay of cell reductive activity. 20 min exposure to visible light and photosensitizer decreased cell viability to ~42%. Lipophilic antioxidants increased viabilities to ~70%, ~61% and ~75% for α-tocopherol, zeaxanthin and their combination, respectively. Cell viabilities were ~70%, 56% and 5% after exposures in the presence of 0.35, 0.7 and 1.4 mM ascorbate, respectively. 45 min exposure increased cell death to ~74% and to >95% in the absence and presence of ascorbate, respectively. In the presence of ascorbate, zeaxanthin did not significantly affect phototoxicity. α-Tocopherol and its combination with zeaxanthin enhanced protective effects of ascorbate but did not prevent from ascorbate-mediated deleterious effects. In conclusion, there is a narrow range of concentrations and exposure times where ascorbate exerts photoprotective effects, exceeding which leads to ascorbate-mediated increase in photocytotoxicity. Vitamin E and its combination with zeaxanthin can enhance protective effects of ascorbate but do not ameliorate its deleterious effects.
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影响因子：
3.3
作者：
Kato, Hisato;Komagoe, Keiko;Katsu, Takashi
通讯作者：
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影响因子：
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影响因子：
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通讯作者：
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作者：
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