Effect of sulfate on low-temperature anaerobic digestion.
Effect of sulfate on low-temperature anaerobic digestion.
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DOI：
10.3389/fmicb.2014.00376
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发表时间：
2014
影响因子：
5.2
通讯作者：
Collins G
中科院分区：
文献类型：
--
作者：
Madden P;Al-Raei AM;Enright AM;Chinalia FA;de Beer D;O'Flaherty V;Collins G
在15°C条件下，研究了硫酸盐对低温厌氧膨胀颗粒污泥床生物反应器稳定性和微生物群落行为的影响。生物反应器表现出高效的性能，化学需氧量（COD）去除率> 90%，平均SO2−4去除率为98.3%。在COD：SO2−4进水比例为8：1和随后的3：1时，原位产甲烷似乎不受影响，只有当COD：SO2−4比例为1：2时才受到轻微影响。特定产甲烷活性测定表明硫酸盐还原菌（SRB）、产甲烷菌和同型产乙酸菌之间存在一系列复杂的相互作用。SO2−4的加入导致了主要的乙酸分解，而不是氢营养，甲烷生成，直到SO2−4影响的生物反应器运行超过600天。通过16S rRNA基因的变性梯度凝胶电泳（DGGE）监测时间微生物群落的发展。荧光原位杂交（FISH），qPCR和微传感器分析相结合，以调查微生物群体的分布，特别是SRB和产甲烷菌，沿着颗粒生物膜的结构。qPCR数据表明，产硫基因存在于产甲烷和产硫生物膜中，表明即使在未暴露于SO2−4的生物反应器中也有硫酸盐还原的潜力。尽管产甲烷颗粒和产硫颗粒的结构相似，表明甚至在产甲烷系统中也存在SRB，但以rRNA为靶标的FISH发现，SRB在产硫生物膜中更丰富。甲烷八叠球菌属物种是古菌群落的主要关键成员，试验结论表明实验生物反应器中完全不存在甲烷八叠球菌属物种。微传感器数据表明，有序分布的硫酸盐还原和硫化物积累，甚至在产甲烷颗粒。
The effect of sulfate addition on the stability of, and microbial community behavior in, low-temperature anaerobic expanded granular sludge bed-based bioreactors was investigated at 15°C. Efficient bioreactor performance was observed, with chemical oxygen demand (COD) removal efficiencies of >90%, and a mean SO2−4 removal rate of 98.3%. In situ methanogensis appeared unaffected at a COD: SO2−4 influent ratio of 8:1, and subsequently of 3:1, and was impacted marginally only when the COD: SO2−4 ratio was 1:2. Specific methanogenic activity assays indicated a complex set of interactions between sulfate-reducing bacteria (SRB), methanogens and homoacetogenic bacteria. SO2−4 addition resulted in predominantly acetoclastic, rather than hydrogenotrophic, methanogenesis until >600 days of SO2−4-influenced bioreactor operation. Temporal microbial community development was monitored by denaturation gradient gel electrophoresis (DGGE) of 16S rRNA genes. Fluorescence in situ hybridizations (FISH), qPCR and microsensor analysis were combined to investigate the distribution of microbial groups, and particularly SRB and methanogens, along the structure of granular biofilms. qPCR data indicated that sulfidogenic genes were present in methanogenic and sulfidogenic biofilms, indicating the potential for sulfate reduction even in bioreactors not exposed to SO2−4. Although the architecture of methanogenic and sulfidogenic granules was similar, indicating the presence of SRB even in methanogenic systems, FISH with rRNA targets found that the SRB were more abundant in the sulfidogenic biofilms. Methanosaeta species were the predominant, keystone members of the archaeal community, with the complete absence of the Methanosarcina species in the experimental bioreactor by trial conclusion. Microsensor data suggested the ordered distribution of sulfate reduction and sulfide accumulation, even in methanogenic granules.
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影响因子：
2.8
作者：
KRISTJANSSON, JK;SCHONHEIT, P;THAUER, RK
通讯作者：
THAUER, RK
影响因子：
4.4
作者：
Akila, G.;Chandra, T. S.
通讯作者：
Chandra, T. S.
影响因子：
2.7
作者：
Lew, B;Tarre, S;Green, M
通讯作者：
Green, M
影响因子：
4.4
作者：
Griffiths, RI;Whiteley, AS;Bailey, MJ
通讯作者：
Bailey, MJ
影响因子：
4.4
作者：
AMANN, RI;STROMLEY, J;STAHL, DA
通讯作者：
STAHL, DA