SAUR15 interaction with BRI1 activates plasma membrane H+-ATPase to promote organ development of Arabidopsis.
SAUR15 interaction with BRI1 activates plasma membrane H+-ATPase to promote organ development of Arabidopsis.
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SAUR15与BRI1相互作用激活质膜H-ATP酶促进拟南芥器官发育
DOI：
10.1093/plphys/kiac194
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发表时间：
2022-08-01
期刊：
影响因子：
7.4
通讯作者：
Yin, Hongju
中科院分区：
文献类型：
--
作者：
Li, Mengzhan;Liu, Chunli;Hepworth, Shelley R.;Ma, Chaofan;Li, Hong;Li, Jia;Wang, Suo-Min;Yin, Hongju
植物甾醇类（BRs）是一类重要的植物甾体激素，对植物的生长发育起着重要的调节作用.小生长素上升RNA（SAUR）家族的几个成员在BR调节的下胚轴伸长和根生长中起作用。然而，其机制尚不清楚。在这里，我们显示在拟南芥（拟南芥），SAUR 15相互作用与细胞表面受体样激酶类油菜素不敏感1（BRI1）在BR处理的植物，导致增强BRI1磷酸化状态和招聘的共同受体BRI1相关受体激酶1。遗传和表型测定表明，SAUR 15对BRI1的作用可以与油菜素甾醇不敏感2活性解偶联。相反，我们发现SAUR15通过磷酸化促进BRI1直接激活质膜H +-ATP酶（PM H +-ATP酶）。因此，SAUR15-BRI1-PM H +-ATP酶作为BR信号传导的直接、基于PM的模式，驱动细胞扩增以促进各种器官的生长和发育。这些数据定义了植物中BR信号传导的替代模式。SAUR15-BRI1-质膜（PM）H +-ATP酶作为油菜素类固醇信号传导的直接、基于PM的模式，驱动细胞扩增以促进植物生长和发育。
Brassinosteroids (BRs) are an important group of plant steroid hormones that regulate growth and development. Several members of the SMALL AUXIN UP RNA (SAUR) family have roles in BR-regulated hypocotyl elongation and root growth. However, the mechanisms are unclear. Here, we show in Arabidopsis (Arabidopsis thaliana) that SAUR15 interacts with cell surface receptor-like kinase BRASSINOSTEROID-INSENSITIVE 1 (BRI1) in BR-treated plants, resulting in enhanced BRI1 phosphorylation status and recruitment of the co-receptor BRI1-ASSOCIATED RECEPTOR KINASE 1. Genetic and phenotypic assays indicated that the SAUR15 effect on BRI1 can be uncoupled from BRASSINOSTEROID INSENSITIVE 2 activity. Instead, we show that SAUR15 promotes BRI1 direct activation of plasma membrane H+-ATPase (PM H+-ATPase) via phosphorylation. Consequently, SAUR15–BRI1–PM H+-ATPase acts as a direct, PM-based mode of BR signaling that drives cell expansion to promote the growth and development of various organs. These data define an alternate mode of BR signaling in plants. SAUR15–BRI1-plasma membrane (PM) H+-ATPase acts as a direct, PM-based mode of brassinosteroid signaling that drives cell expansion to promote plant growth and development.
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影响因子：
7.2
作者：
Kim, GT;Fujioka, S;Tsukaya, H
通讯作者：
Tsukaya, H
影响因子：
64.8
作者：
Li L;Verstraeten I;Roosjen M;Takahashi K;Rodriguez L;Merrin J;Chen J;Shabala L;Smet W;Ren H;Vanneste S;Shabala S;De Rybel B;Weijers D;Kinoshita T;Gray WM;Friml J
通讯作者：
Friml J
影响因子：
64.8
作者：
Lin W;Zhou X;Tang W;Takahashi K;Pan X;Dai J;Ren H;Zhu X;Pan S;Zheng H;Gray WM;Xu T;Kinoshita T;Yang Z
通讯作者：
Yang Z
影响因子：
7.4
作者：
Cho, Daeshik;Villiers, Florent;Kwak, June M.
通讯作者：
Kwak, June M.
影响因子：
64.5
作者：
Li, J;Wen, JQ;Walker, JC
通讯作者：
Walker, JC