Minimal effects of waterborne exposure to single-walled carbon nanotubes on behaviour and physiology of juvenile rainbow trout (Oncorhynchus mykiss).
Minimal effects of waterborne exposure to single-walled carbon nanotubes on behaviour and physiology of juvenile rainbow trout (Oncorhynchus mykiss).
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水中暴露于单壁碳纳米管对虹鳟幼鱼（Oncorhynchus mykiss）行为和生理的影响极小。
DOI：
10.1016/j.aquatox.2013.11.006
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Boyle D
中科院分区：
文献类型：
--
作者：
Boyle D
鱼类的行为通常被认为是水生污染物的敏感端点，但很少有人关注工程纳米材料。本研究旨在确定虹鳟鱼（Oncorhynchus mykiss）在水中暴露于单壁碳纳米管（SWCNTs）时的运动和社会行为，并确定任何观察到的影响的生理基础。在阴离子表面活性剂十二烷基硫酸钠（SDS）中以等w/w的比例搅拌制备SWCNTs分散的原液悬浮液。将鳟鱼分别暴露于对照组（不含SWCNT或SDS）、0.25 mg L - 1SDS（分散剂对照）或0.25 mg L - 1 SWCNT中10天。对单个鱼自发运动的视频跟踪分析显示，swcnts对活动时的平均速度、总移动距离或游动速度分布没有显著影响。肝糖原水平也未受影响。暴露于SWCNTs的鱼在被迫与来自两个对照组的鱼进行能量昂贵的侵略性相互作用时，仍保持竞争适应性。鱼的呼吸生理学评估显示，通气率或鳃损伤没有显著变化。血液中的红细胞压积和血红蛋白浓度不受swcnts暴露的影响；脾脏的红髓和白髓没有变化，排除了保护循环的代偿性造血反应。尽管与对照组相比，暴露于swcnts的鱼的肾脏发生了一些轻微的组织学变化，但等离子体离子浓度和组织电解质在很大程度上未受影响。swcnts的直接神经毒性不太可能，因为大脑显示大部分正常组织学，并且对全脑匀浆中的乙酰胆碱酯酶或Na+/K+- atp酶活性没有影响。本研究中观察到的水媒swcnts暴露的最小影响与我们之前关于鳟鱼swcnts毒性的报告相反，这表明分散方法的细节和分散剂的共同暴露浓度可能会改变毒性。
Fish behaviours are often considered to be sensitive endpoints of waterborne contaminants, but little attention has been given to engineered nanomaterials. The present study aimed to determine the locomotor and social behaviours of rainbow trout (Oncorhynchus mykiss) during waterborne exposure to single-walled carbon nanotubes (SWCNTs), and to ascertain the physiological basis for any observed effects. Dispersed stock suspensions of SWCNTs were prepared by stirring in sodium dodecyl sulphate (SDS), an anionic surfactant, on an equal w/w basis. Trout were exposed to control (no SWCNT or SDS), 0.25 mg L−1SDS (dispersant control), or 0.25 mg L−1of SWCNT for 10 days. Video tracking analysis of spontaneous locomotion of individual fish revealed no significant effects of SWCNT on mean velocity when active, total distance moved, or the distribution of swimming speeds. Hepatic glycogen levels were also unaffected. Fish exposed to SWCNTs retained competitive fitness when compelled to compete in energetically costly aggressive interactions with fish from both control groups. Assessment of the respiratory physiology of the fish revealed no significant changes in ventilation rate or gill injuries. Haematocrit and haemoglobin concentrations in the blood were unaffected by SWCNT exposure; and the absence of changes in the red and white pulp of the spleen excluded a compensatory haematopoietic response to protect the circulation. Despite some minor histological changes in the kidneys of fish exposed to SWCNT compared to controls, plasma ion concentrations and tissue electrolytes were largely unaffected. Direct neurotoxicity of SWCNT was unlikely with the brains showing mostly normal histology, and with no effects on acetylcholinesterase or Na+/K+-ATPase activities in whole brain homogenates. The minimal effects of waterborne exposure to SWCNT observed in this study are in contrast to our previous report of SWCNT toxicity in trout, suggesting that details of the dispersion method and co-exposure concentration of the dispersing agent may alter toxicity.
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影响因子：
4.5
作者：
Alicia A. Taylor;G. M. Aron;G. Beall;N. Dharmasiri;Yixin Zhang;R. McLean
通讯作者：
Alicia A. Taylor;G. M. Aron;G. Beall;N. Dharmasiri;Yixin Zhang;R. McLean
影响因子：
4.5
作者：
B. J. Shaw;G. Al-Bairuty;R. Handy
通讯作者：
B. J. Shaw;G. Al-Bairuty;R. Handy
影响因子：
4.5
作者：
Smith, Catherine J.;Shaw, Benjamin J.;Handy, Richard D.
通讯作者：
Handy, Richard D.
影响因子：
2
作者：
HANDY, RD;EDDY, FB
通讯作者：
EDDY, FB
影响因子：
2
作者：
ABEL, PD
通讯作者：
ABEL, PD