Multiple Regulatory Networks Are Activated during Cold Stress in Medicago sativa L.
Multiple Regulatory Networks Are Activated during Cold Stress in Medicago sativa L.
复制标题
紫花苜蓿在冷应激过程中多个调控网络被激活。
DOI：
10.3390/ijms19103169
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发表时间：
2018-10-15
影响因子：
5.6
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Zhou Q;Luo D;Chai X;Wu Y;Wang Y;Nan Z;Yang Q;Liu W;Liu Z
栽培苜蓿（Medicago sativa L.）豆科牧草是世界上最重要的多年生豆科牧草之一，具有很大的发展潜力。然而，苜蓿对冷胁迫反应的分子机制在很大程度上是未知的。在这项研究中，苜蓿在4 °C冷胁迫下2，6，24和48 h（每个时间点重复三次）的转录组变化使用高通量测序平台BGISEQ-500进行了分析，鉴定了50，809个注释的unigenes和5283个差异表达基因（DEG）。代谢途径富集分析表明，DEGs参与碳水化合物代谢、光合作用、植物激素信号转导和氨基酸的生物合成。此外，谷胱甘肽和脯氨酸含量，过氧化氢酶和过氧化物酶活性的生理变化与相关基因的动态转录谱相一致。此外，低温胁迫处理48 h后相关基因的表达谱分析表明，一些转录因子可能在紫花苜蓿对低温胁迫的响应中起重要作用。这些发现为鉴定和表征苜蓿冷信号网络中的重要组分提供了有价值的信息，并加深了对苜蓿冷胁迫响应的分子机制的理解。
Cultivated alfalfa (Medicago sativa L.) is one of the most important perennial legume forages in the world, and it has considerable potential as a valuable forage crop for livestock. However, the molecular mechanisms underlying alfalfa responses to cold stress are largely unknown. In this study, the transcriptome changes in alfalfa under cold stress at 4 °C for 2, 6, 24, and 48 h (three replicates for each time point) were analyzed using the high-throughput sequencing platform, BGISEQ-500, resulting in the identification of 50,809 annotated unigenes and 5283 differentially expressed genes (DEGs). Metabolic pathway enrichment analysis demonstrated that the DEGs were involved in carbohydrate metabolism, photosynthesis, plant hormone signal transduction, and the biosynthesis of amino acids. Moreover, the physiological changes of glutathione and proline content, catalase, and peroxidase activity were in accordance with dynamic transcript profiles of the relevant genes. Additionally, some transcription factors might play important roles in the alfalfa response to cold stress, as determined by the expression pattern of the related genes during 48 h of cold stress treatment. These findings provide valuable information for identifying and characterizing important components in the cold signaling network in alfalfa and enhancing the understanding of the molecular mechanisms underlying alfalfa responses to cold stress.
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