Dynamics of CaCdc10, a septin of Candida albicans, in living cells and during infection.

Dynamics of CaCdc10, a septin of Candida albicans, in living cells and during infection.
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CaCdc10(白色念珠菌的一种隔膜)在活细胞中和感染过程中的动态。

DOI:
10.2436/im.v7i2.9454
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发表时间:
2004
期刊:
International microbiology : the official journal of the Spanish Society for Microbiology
影响因子:
--
通讯作者:
M. Sánchez
M. Sánchez
中科院分区:
--
文献类型:
--
作者:
Alberto González;J. Jiménez;M. García;Inmaculada Ríos;J. Pla;Antonio Jiménez;M. Sánchez

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致病真菌白念珠菌的形态发生程序,包括二态转变,是一个有趣的研究领域,不仅因为它在常用的模式酵母酿酒酵母中是不存在的,而且因为白念珠菌的菌丝发育与毒力之间存在着密切的关系。我们研究了真菌形态发生中最重要的一个方面--白念珠菌中的隔膜环。通过绿色荧光蛋白(GFP)的融合构建,对白色念珠菌CDC10在不同形态中的亚细胞定位和动态进行了鉴定。将所获得的定位特征与直接从动物感染模型中提取的念珠菌细胞在尽可能类似于白色念珠菌感染宿主期间的生理条件的环境条件下获得的结果进行了对比和比较。
The morphogenetic program in the pathogenic fungus Candida albicans, including the dimorphic transition, is an interesting field of study, not only because it is absent in the commonly used model yeast Saccharomyces cerevisiae, but because of the close relationship between hyphal development and virulence of C. albicans. We studied one of the most important aspects of fungal morphogenesis--the septin ring--in C. albicans. By using a fusion construct to green fluorescent protein (GFP), the subcellular localization and dynamics of C. albicans Cdc10 in the different morphologies that this fungus is able to adopt was identified. The localization features reached were contrasted and compared with the results obtained from Candida cells directly extracted from an animal infection model under environmental conditions as similar as possible to the physiological conditions encountered by C. albicans during host infection.
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影响因子: 4
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