Developmental expression of G-proteins and adenylyl cyclase in peripheral olfactory systems. Light microscopic and freeze-substitution electron microscopic immunocytochemistry
Developmental expression of G-proteins and adenylyl cyclase in peripheral olfactory systems. Light microscopic and freeze-substitution electron microscopic immunocytochemistry
复制标题
外周嗅觉系统中 G 蛋白和腺苷酸环化酶的发育表达。
DOI：
10.1007/bf01181645
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
W. Danho
中科院分区：
文献类型：
--
作者：
B. Menco;F. Tekula;A. Farbman;W. Danho
摘要：采用光镜免疫组织化学联合冷冻取代电镜免疫细胞化学技术对发育中的大鼠嗅上皮组织中g蛋白α-亚基和III型腺苷酸环化酶进行了定位。一些纤毛早在产前15天就与Gsα和III型腺苷酸环化酶抗体发生免疫反应（E15; El=精子阳性），但直到E16才观察到与Golfα抗体的免疫标记。从那时起，具有免疫标记纤毛的受体细胞数量在所有三种探针中都有所增加。嗅觉信号转导蛋白抗体的免疫反应性倾向于与纤毛发育平行，尽管Golfα在一定程度上滞后。新形成的纤毛沿着它们的长度标记，而成熟的纤毛主要沿着它们的长远端标记。树突状旋钮和纤毛状项链显示很少或没有标记。而在E22中，大多数多翼细胞都有针对Gsα、Golfα和III型腺苷酸环化酶的抗体，并不是所有这些细胞都有针对嗅觉标记蛋白的抗体。嗅觉轴突对gsa α抗体的免疫反应比上皮表面结构更强烈；相反的情况发生在E18左右。早在E15就发现了g蛋白Go、Gq/G11和Gi的a亚基抗体的免疫反应性。仅在发育过程中针对α α标记受体细胞树突状突起和纤毛的抗体。针对Gqα的抗体标记鲍曼腺，而针对Gqα/ g11 α的抗体结合受体细胞纤毛和轴突（主要是表鼻），以及支持细胞微绒毛。我们认为G蛋白在未成熟嗅觉受体细胞的纤毛中占优势，而Goif蛋白在成熟细胞的纤毛中占优势。某些g蛋白抗体的轴突免疫反应性表明g蛋白参与了嗅觉轴突和/或轴突末端结合信号的加工。
SummaryLight microscopic immunohistochemistry coupled with freeze-substitution electron microscopic immunocytochemistry was used to localize α-subunits of G-proteins and type III adenylyl cyclase in developing rat olfactory epithelia. Some cilia immunoreacted with antibodies to Gsα and type III adenylyl cyclase as early as prenatal day 15 (E15; El=sperm-positive), but immunolabelling with antibodies to Golfα was not observed until E16. From then on numbers of receptor cells with immunolabelled cilia increased for all three probes. Immunoreactivity for antibodies to the olfactory signal-transduction proteins tended to parallel cilium development, though Golfα lags somewhat behind. Newly formed cilia labelled along their lengths, whereas mature cilia labelled predominantly along their long distal parts. Dendritic knobs and ciliary necklaces showed little or no labelling. While at E22 most multiciliate cells immunolabelled with antibodies to Gsα, Golfα, and type III adenylyl cyclase, not all of these cells labelled with antibodies to olfactory marker protein. Olfactory axons immunoreacted more intensely than epithelial surface structures with antibodies to Gsα at E15; the reverse occurred by about E18. Immunoreactivity with antibodies to a-subunits of the G-proteins Go, Gq/G11, and Gi was also found as early as E15. Antibodies to Goα labelled receptor cell dendritic knobs and cilia during development only. Antibodies to Giα labelled Bowman's glands, whereas those to Gqα/G11αbound to receptor cell cilia and axons (primarily vomeronasal), and supporting cell microvilli. We propose that Gs is the predominant G protein in cilia of immature olfactory receptor cells, while Goif is predominant in cilia of mature cells. Axonal immunoreactivity for some G-protein antibodies suggests G-protein participation in processing of olfactory axon and/or axon terminal-bound signals.
登录
查看更多内容
从牛中枢神经系统中分离出的 GTP 结合蛋白家族内部的关系。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Roof,DJ;Applebury,ML;Sternweis,PC
通讯作者：
Sternweis,PC
分离的大鼠嗅觉纤毛中存在 InsP3 门控通道蛋白的证据。
DOI：
10.1152/ajpcell.1992.263.3.c667
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
--
作者：
Restrepo,D;Teeter,JH;Honda,E;Boyle,AG;Marecek,JF;Prestwich,GD;Kalinoski,DL
通讯作者：
Kalinoski,DL
影响因子：
56.9
作者：
SMRCKA, AV;HEPLER, JR;STERNWEIS, PC
通讯作者：
STERNWEIS, PC
影响因子：
2.9
作者：
CHOI, EJ;XIA, ZG;STORM, DR
通讯作者：
STORM, DR
影响因子：
56.9
作者：
KRUPINSKI, J;COUSSEN, F;GILMAN, AG
通讯作者：
GILMAN, AG