Evidence for an InsP3-gated channel protein in isolated rat olfactory cilia.
Evidence for an InsP3-gated channel protein in isolated rat olfactory cilia.
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分离的大鼠嗅觉纤毛中存在 InsP3 门控通道蛋白的证据。
DOI：
10.1152/ajpcell.1992.263.3.c667
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Kalinoski,DL
中科院分区：
文献类型：
--
作者：
Restrepo,D;Teeter,JH;Honda,E;Boyle,AG;Marecek,JF;Prestwich,GD;Kalinoski,DL
刺激大鼠嗅觉纤毛（ROC）与气味导致的cAMP或三磷酸肌醇（InsP 3）的水平的瞬时升高。我们的特点是结合[3 H] InsP 3孤立的ROC。未标记的InsP 3以剂量依赖性方式取代[3 H] InsP 3结合（解离常数= 3.9 +/- 0.65 μ M）。结合是立体特异性的，并依赖于肌醇环中磷酸的数量。在125 I标记的InsP 3类似物1-O-[N-（4-叠氮水杨氧基）-3-氨基丙基-1-磷酸]-肌醇4，5-二磷酸存在下，将纤毛膜暴露于紫外光后，特异性标记分子量为120 kDa的纤毛蛋白。该蛋白的标记显示与[3 H] InsP 3结合ROC的立体特异性相同。此外，ROC膜纳入磷脂双层贴片移液管尖端显示电导增加后，暴露于微摩尔D-肌醇1，4，5-三磷酸在45%的试验（n = 88）。InsP 3门控电导对于阳离子是相对非特异性的，并且与cAMP门控电导不同。电导显示与InsP 3结合实验一致的立体特异性。结果表明，气味刺激大鼠嗅纤毛中InsP 3浓度升高的作用部位是纤毛InsP 3门控通道。
Stimulation of rat olfactory cilia (ROC) with odorants leads to a transient elevation in the levels of either cAMP or inositol trisphosphate (InsP3). We have characterized the binding of [3H]InsP3 to isolated ROC. Unlabeled InsP3 displaced [3H]InsP3 binding in a dose-dependent manner (dissociation constant = 3.9 +/- 0.65 microM). Binding was stereospecific and dependent on the number of phosphates in the inositol ring. A ciliary protein of 120 kDa molecular mass was labeled specifically upon exposure of cilia membranes to ultraviolet light in the presence of the 125I-labeled InsP3 analogue 1-O-[N-(4-azidosaliciloxy)-3-aminopropyl-1-phospho]-myo-inositol 4,5-bisphosphate. Labeling of this protein displayed the same stereospecificity as binding of [3H]InsP3 to ROC. In addition, ROC membranes incorporated into a phospholipid bilayer at the tip of a patch pipette displayed an increase in conductance upon exposure to micromolar D-myoinositol 1,4,5-trisphosphate in 45% of the trials (n = 88). The InsP3-gated conductance is relatively nonspecific for cations and is distinct from the cAMP-gated conductance. The conductance displayed stereospecificity consistent with the InsP3 binding experiments. The results suggest that the site of action for odorant-stimulated elevations in InsP3 concentration in rat olfactory cilia is at a ciliary InsP3-gated channel.
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影响因子：
15
作者：
G. Prestwich;J. Marecek;R. Mourey;A. Theibert;C. D. Ferris;S. Danoff;S. Snyder
通讯作者：
S. Snyder
影响因子：
3.5
作者：
BRUCH, RC;TEETER, JH
通讯作者：
TEETER, JH
气味敏感的腺苷酸环化酶：在原代嗅觉神经元培养物中快速、有效的激活和脱敏。
DOI：
10.1073/pnas.88.6.2366
发表时间：
1991
影响因子：
11.1
作者：
Ronnett,GV;Parfitt,DJ;Hester,LD;Snyder,SH
通讯作者：
Snyder,SH
影响因子：
3.5
作者：
BREER, H;BOEKHOFF, I
通讯作者：
BOEKHOFF, I
影响因子：
3.5
作者：
LUDWIG, J;MARGALIT, T;KAUPP, UB
通讯作者：
KAUPP, UB