Mass spectrometry-based characterization of the vitreous phosphoproteome.
Mass spectrometry-based characterization of the vitreous phosphoproteome.
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DOI：
10.1002/prca.201000032
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发表时间：
2010-11
影响因子：
2
通讯作者：
Zhou, Weidong
中科院分区：
文献类型：
--
作者：
Tamburro, Davide;Facchiano, Francesco;Petricoin, Emanuel F.;Liotta, Lance A.;Zhou, Weidong
玻璃状凝胶是含有许多蛋白质的高度水合的细胞外基质。这些蛋白质可能通过局部分泌、从血液中过滤或从周围组织和脉管系统扩散而积聚在玻璃体中，并且可能在疾病状态下改变。在过去的几年中，一些大规模的玻璃体蛋白质谱的报告已经出版，但是，有很少的信息玻璃体的磷酸化蛋白质组的表征。在这里，我们试图从玻璃体中识别磷酸肽及其磷酸化位点。采用二氧化钛（TiO 2）富集玻璃体中的磷酸肽，并通过液相色谱-串联质谱（LC-MS/MS）进行鉴定。结果：从44个蛋白质中鉴定出85个独特的磷酸肽和磷酸化位点。我们提出了一种方法，利用目前的质谱技术，从玻璃体样品的磷酸化蛋白质组的表征，并产生了初步评估的磷蛋白/肽含量的人玻璃体，从而提供重要的生物学信息，进一步了解翻译后修饰的玻璃体蛋白质及其在疾病中的功能意义。
The vitreous gel is a highly hydrated extracellular matrix containing many proteins. These proteins are likely accumulated in the vitreous by local secretion, filtration from the blood, or diffusion from the surrounding tissues and vasculature, and may be altered in disease state. In the last several years, several reports of large-scale profiling of vitreous proteins have been published; however, there is little information on the characterization of the phosphoproteome of vitreous. Here, we sought to identify phosphopeptides and their phosphorylation sites from vitreous. We used titanium dioxide (TiO2) to enrich phosphopeptides from vitreous and identified them by liquid chromatography coupled tandem mass spectrometry (LC-MS/MS). Results: We identified 85 unique phosphopeptides and the phosphorylation sites from 44 proteins. We present a method for characterization of phosphoproteome from vitreous samples using current mass spectrometry technologies and yielded an initial assessment of the phosphoprotein/peptide content of human vitreous, thus providing important biological information toward further understanding of the post-translational modifications of vitreous proteins and their functional significance in disease.
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影响因子：
4.4
作者：
Bahk, Song-Chul;Jang, Jung-Un;Chung, Hun-Taeg
通讯作者：
Chung, Hun-Taeg
DOI：
10.1016/0006-291x(87)91457-4
发表时间：
1987-05-14
影响因子：
3.1
作者：
CHIESA, R;GAWINOWICZKOLKS, MA;SPECTOR, A
通讯作者：
SPECTOR, A
影响因子：
3.4
作者：
HADDAD, A;DEALMEIDA, JC;PELLETIER, G
通讯作者：
PELLETIER, G
影响因子：
--
作者：
Davuluri, Geetanjali;Espina, Virginia;Glaser, Bert M.
通讯作者：
Glaser, Bert M.
影响因子：
3.4
作者：
HADDAD, A;LAICINE, EM;COSTA, MSA
通讯作者：
COSTA, MSA