Streptococcus suis DivIVA Protein Is a Substrate of Ser/Thr Kinase STK and Involved in Cell Division Regulation.
Streptococcus suis DivIVA Protein Is a Substrate of Ser/Thr Kinase STK and Involved in Cell Division Regulation.
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猪链球菌 DivIVA 蛋白是 Ser/Thr 激酶 STK 的底物并参与细胞分裂调节
DOI：
10.3389/fcimb.2018.00085
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发表时间：
2018
影响因子：
5.7
通讯作者：
Pan X
中科院分区：
文献类型：
--
作者：
Ni H;Fan W;Li C;Wu Q;Hou H;Hu D;Zheng F;Zhu X;Wang C;Cao X;Shao ZQ;Pan X
Streptococcus suis serotype 2 is an important swine pathogen and an emerging zoonotic agent that causes severe infections. Recent studies have reported a eukaryotic-like Ser/Thr protein kinase (STK) gene and characterized its role in the growth and virulence of different S. suis 2 strains. In the present study, phosphoproteomic analysis was adopted to identify substrates of the STK protein. Seven proteins that were annotated to participate in different cell processes were identified as potential substrates, which suggests the pleiotropic effects of stk on S. suis 2 by targeting multiple pathways. Among them, a protein characterized as cell division initiation protein (DivIVA) was further investigated. In vitro analysis demonstrated that the recombinant STK protein directly phosphorylates threonine at amino acid position 199 (Thr-199) of DivIVA. This effect could be completely abolished by the T199A mutation. To determine the specific role of DivIVA in growth and division, a divIVA mutant was constructed. The ΔdivIVA strain exhibited impaired growth and division, including lower viability, enlarged cell mass, asymmetrical division caused by aberrant septum, and extremely weak pathogenicity in a mouse infection model. Collectively, our results reveal that STK regulates the cell growth and virulence of S. suis 2 by targeting substrates that are involved in different biological pathways. The inactivation of DivIVA leads to severe defects in cell division and strongly attenuates pathogenicity, thereby indicating its potential as a molecular drug target against S. suis.
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影响因子：
2.8
作者：
de Buhr, Nicole;Neumann, Ariane;Baums, Christoph G.
通讯作者：
Baums, Christoph G.
影响因子：
3.7
作者：
Chen C;Tang J;Dong W;Wang C;Feng Y;Wang J;Zheng F;Pan X;Liu D;Li M;Song Y;Zhu X;Sun H;Feng T;Guo Z;Ju A;Ge J;Dong Y;Sun W;Jiang Y;Wang J;Yan J;Yang H;Wang X;Gao GF;Yang R;Wang J;Yu J
通讯作者：
Yu J
猪链球菌2型丝氨酸/苏氨酸磷酸酶1是重要的毒力因子
影响因子：
1.8
作者：
Fang L;Zhou J;Fan P;Yang Y;Shen H;Fang W
通讯作者：
Fang W
影响因子：
4.8
作者：
Burnett, Benjamin J.;Altman, Roger B.;Blanchard, Scott C.
通讯作者：
Blanchard, Scott C.
影响因子：
3.1
作者：
Echenique, J;Kadioglu, A;Trombe, MC
通讯作者：
Trombe, MC