Biodistribution Study of Nanoparticle Encapsulated Photodynamic Therapy Drugs Using Multispectral Imaging.
Biodistribution Study of Nanoparticle Encapsulated Photodynamic Therapy Drugs Using Multispectral Imaging.
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DOI：
10.1117/12.2006492
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发表时间：
2013-03-29
期刊：
影响因子：
--
通讯作者：
Fei B
中科院分区：
文献类型：
--
作者：
Halig LV;Wang D;Wang AY;Chen ZG;Fei B
光动力疗法（PDT）使用一种称为光敏剂的药物，该药物通过特定波长的激光照射而激发，该激光产生破坏肿瘤细胞的活性单线态氧。光敏剂和光是惰性的;因此，PDT中的全身毒性最小化。新型PDT药物的合成和光敏剂纳米载体的使用可以提高治疗和药物递送的效率。在这项研究中，我们制定了两个纳米粒子与和没有一个靶向配体封装酞菁4（Pc 4）分子，并比较其生物分布。转移性人头颈癌细胞（M4 e）移植到裸鼠体内。2-3周后，小鼠注射Pc 4、包封在表面包被的氧化铁中的Pc 4（IO-Pc 4）和与特异性结合整合素β1的纤连蛋白模拟肽缀合的IO-Pc 4（FMP-IO-Pc 4）。使用多光谱照相机对小鼠进行成像。使用多光谱图像，创建光谱特征库，并计算每个肿瘤的每像素信号，以每种药物的未混合信号的灰度表示。观察到与非配制的Pc 4相比，纳米颗粒包封的PDT药物的生物分布增强。此外，包裹Pc 4的特异性靶向纳米颗粒比非靶向纳米颗粒具有更快的递送时间和在肿瘤组织中的积累。纳米颗粒封装的PDT药物可以在癌症成像和治疗中具有各种潜在的应用。
Photodynamictherapy (PDT) uses a drug called a photosensitizer that is excited by irradiation with a laser light of a particular wavelength, which generates reactive singlet oxygen that damages the tumor cells. The photosensitizer and light are inert; therefore, systemic toxicities are minimized in PDT. The synthesis of novel PDT drugs and the use of nanosized carriers for photosensitizers may improve the efficiency of the therapy and the delivery of the drug. In this study, we formulated two nanoparticles with and without a targeting ligand to encapsulate phthalocyanines 4 (Pc 4) molecule and compared their biodistributions. Metastatic human head and neck cancer cells (M4e) were transplanted into nude mice. After 2–3 weeks, the mice were injected with Pc 4, Pc 4 encapsulated into surface coated iron oxide (IO-Pc 4), and IO-Pc 4 conjugated with a fibronectin-mimetic peptide (FMP-IO-Pc 4) which binds specifically to integrin β1. The mice were imaged using a multispectral camera. Using multispectral images, a library of spectral signatures was created and the signal per pixel of each tumor was calculated, in a grayscale representation of the unmixed signal of each drug. An enhanced biodistribution of nanoparticle encapsulated PDT drugs compared to non-formulated Pc 4 was observed. Furthermore, specific targeted nanoparticles encapsulated Pc 4 has a quicker delivery time and accumulation in tumor tissue than the non-targeted nanoparticles. The nanoparticle-encapsulated PDT drug can have a variety of potential applications in cancer imaging and treatment.
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影响因子：
82.9
作者：
Lee, Jae-Hyun;Huh, Yong-Min;Cheon, Jinwoo
通讯作者：
Cheon, Jinwoo
影响因子：
10.8
作者：
Gaucher, G;Dufresne, MH;Leroux, JC
通讯作者：
Leroux, JC
影响因子：
158.5
作者：
Forastiere, AA;Goepfert, H;Cooper, J
通讯作者：
Cooper, J
DOI：
10.1016/j.jconrel.2012.01.045
发表时间：
2012-05-10
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Rahman MA;Amin AR;Wang X;Zuckerman JE;Choi CH;Zhou B;Wang D;Nannapaneni S;Koenig L;Chen Z;Chen ZG;Yen Y;Davis ME;Shin DM
通讯作者：
Shin DM
影响因子：
2.4
作者：
Baran, Timothy M.;Giesselman, Benjamin R.;Hu, Rui;Biel, Merrill A.;Foster, Thomas H.
通讯作者：
Foster, Thomas H.