The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA.
The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA.
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DOI：
10.1080/10253890.2018.1446518
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Pace TWW
中科院分区：
文献类型：
--
作者：
Quinn AM;Williams AR;Sivilli TI;Raison CL;Pace TWW
白细胞介素（IL）-6的循环浓度是一种炎症生物标志物，在人类中广泛评估，以研究急性心理应激的炎症反应，几十年来一直使用酶联免疫吸附测定（ELISA）进行定量。然而，生物行为研究人员越来越多地使用细胞因子多重测定代替ELISA来测量IL-6和其他细胞因子。尽管有这种趋势，多重测定法尚未直接与ELISA法比较其检测IL-6的细微应激诱导变化的能力。在这里，我们测试了高灵敏度多重测定（人磁性Luminex性能测定，R&D Systems）将以与高灵敏度ELISA相当的方式检测作为急性应激攻击的结果的IL-6的变化的预测。特里尔社会应激试验（TSST）是一种急性实验室心理社会应激挑战，在开始前即刻以及开始后90分钟和210分钟，从12名健康成人中采集血液。除了用多重和ELISA定量血浆中的IL-6浓度之外，我们还用多重评估了肿瘤坏死因子-α、IL-8、IL-10、IL-5和IL-2的浓度。多重检测所有样品中的IL-6。在所有样品中以及在各个TSST时间点收集的样品中，浓度与通过ELISA测定的值强烈相关（r=0.941，p<0.001）。通过多重和ELISA捕获的对TSST的IL-6应答（即曲线下面积）也是强相关的（rs =0.937，p<0.001）。虽然通过多重检测检测了其他细胞因子，但在检查的时间点，TSST攻毒未导致任何变化。这些结果表明，高灵敏度的磁性多重测定能够检测IL-6的血浆浓度的变化，作为人类急性应激的结果。
Circulating concentrations of interleukin (IL)-6, an inflammatory biomarker widely assessed in humans to study the inflammatory response to acute psychological stress, have for decades been quantified using enzyme-linked immunosorbent assay (ELISA). However, biobehavioral researchers are increasingly using cytokine multiplex assays instead of ELISA to measure IL-6 and other cytokines. Despite this trend, multiplex assays have not been directly compared to ELISA for their ability to detect subtle stress-induced changes of IL-6. Here we tested the prediction that a high-sensitivity multiplex assay (human Magnetic Luminex Performance Assay, R&D Systems) would detect changes in IL-6 as a result of acute stress challenge in a manner comparable to high-sensitivity ELISA. Blood was collected from 12 healthy adults immediately before and then 90 and 210 minutes after the start of the Trier Social Stress Test (TSST), an acute laboratory psychosocial stress challenge. In addition to quantifying IL-6 concentrations in plasma with both multiplex and ELISA, we also assessed concentrations of tumor necrosis factor-alpha, IL-8, IL-10, IL-5, and IL-2 with multiplex. The multiplex detected IL-6 in all samples. Concentrations strongly correlated with values determined by ELISA across all samples (r=0.941, p<0.001) as well as among samples collected at individual TSST time points. IL-6 responses to the TSST (i.e. area under the curve) captured by multiplex and ELISA were also strongly correlated (rs =0.937, p<0.001). While other cytokines were detected by multiplex, none changed as a result of TSST challenge at time points examined. These results suggest high-sensitivity magnetic multiplex assay is able to detect changes in plasma concentrations of IL-6 as a result of acute stress in humans.
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影响因子：
15.1
作者：
Fagundes, Christopher P.;Glaser, Ronald;Hwang, Beom Seuk;Malarkey, William B.;Kiecolt-Glaser, Janice K.
通讯作者：
Kiecolt-Glaser, Janice K.
影响因子：
4.4
作者：
Rosenberg-Hasson, Yael;Hansmann, Leo;Liedtke, Michaela;Herschmann, Iris;Maecker, Holden T.
通讯作者：
Maecker, Holden T.
影响因子：
3.7
作者：
Pruessner, JC;Kirschbaum, C;Hellhammer, DH
通讯作者：
Hellhammer, DH
影响因子：
3.2
作者：
KIRSCHBAUM, C;PIRKE, KM;HELLHAMMER, DH
通讯作者：
HELLHAMMER, DH
影响因子：
5.5
作者：
de Brouwer, Sabine J. M.;van Middendorp, Henriet;Evers, Andrea W. M.
通讯作者：
Evers, Andrea W. M.