Phosphorus magnesium fiber regulates macrophage polarization through TRPM7 to accelerate wound healing
Phosphorus magnesium fiber regulates macrophage polarization through TRPM7 to accelerate wound healing
复制标题
磷镁纤维通过TRPM7调节巨噬细胞极化加速伤口愈合
DOI：
10.1016/j.apmt.2023.101758
复制
发表时间：
2023-04
影响因子：
8.3
通讯作者：
Hongyu Zhang
中科院分区：
文献类型：
--
作者：
xuebo wei;Zhiyong Liao;Liangliang Yang;Fangfang Wu;Shaodong Chen;Chuxiao Shao;Xin Wang;Keshen Xiao;Ke Xu;Da Sun;Hongyu Zhang
伤口愈合是一个复杂的过程，创伤后持续的炎症反应可能会延迟伤口愈合。许多研究发现，原位应用生物材料可释放金属离子进入创面，促进创面愈合，但其机制尚未阐明。因此，我们开发了一种新型纳米功能材料磷镁纤维（PMF）作为伤口敷料，以探索其与伤口愈合的关系。本研究中，体内创面愈合结果显示，PMF在全层创面动物模型中加速创面闭合，伴有胶原沉积和肉芽形成。PMF通过向创面长期释放镁离子，调控TRPM7-ERK1/2信号，加速巨噬细胞极化，促进创面床细胞增殖、生长因子生成和ECM合成，最终促进创面愈合。体外研究表明，PMF可促进巨噬细胞极化至抗炎M2表型。同时，PMF上调TRPM7-ERK1/2的表达。总之，我们的研究证实了PMF与TRPM7通道蛋白介导的巨噬细胞行为之间的关系，并随后研究了PMF在伤口愈合中的功能。这种镁基纳米纤维材料提供了一种治疗开放性伤口的创新方法，有望在组织再生领域用作伤口敷料。
Wound healing is a complex process that can be delayed by persistent inflammatory responses after trauma. Many studies have found that in situ application of biomaterials releases metal ions into wounds to promote wound healing, but the underlying mechanism has not been elucidated. Therefore, we developed a novel nanoscale functional material, phosphorus magnesium fiber (PMF), as a wound dressing to explore its association with wound healing. In this study, in vivo wound healing results showed that PMF accelerated wound closure in full-thickness wound animal models, accompanied by collagen deposition and granulation formation. Moreover, PMF accelerated macrophage polarization by regulating TRPM7-ERK1/2 signaling through the long-term release of magnesium ions into the wound, which contributes to enhancing cell proliferation, growth factor production, and synthesis of ECM in the wound bed, ultimately facilitating wound healing. In vitro studies revealed that PMF promoted the polarization of macrophages to the anti-inflammatory M2 phenotype. Meanwhile, PMF up-regulated TRPM7-ERK1/2 expression. In conclusion, our study confirmed the relationship between PMF and macrophage behavior mediated by the TRPM7 channel protein and subsequently investigated the function of PMF in wound healing. This magnesium-based nanofiber material offers an innovative method for treating open wounds and is anticipated to be used as a wound dressing in the field of tissue regeneration.
登录
查看更多内容
影响因子：
29
作者：
Runtsch, Marah C.;Angiari, Stefano;O'Neill, Luke A. J.
通讯作者：
O'Neill, Luke A. J.
影响因子：
9.5
作者：
Qijuan Yuan;Jun Huang;Caihong Xian;Jun Wu
通讯作者：
Qijuan Yuan;Jun Huang;Caihong Xian;Jun Wu
影响因子：
9.3
作者：
O. Yazarlu;M. Iranshahi;Hamid Reza Khayat Kashani;Sara Reshadat;S. Habtemariam;Milad Iranshahy;Maede Hasanpour
通讯作者：
O. Yazarlu;M. Iranshahi;Hamid Reza Khayat Kashani;Sara Reshadat;S. Habtemariam;Milad Iranshahy;Maede Hasanpour
合理设计的蛋白质交联水凝胶通过协同释放镁和锌离子实现骨再生
影响因子：
14
作者：
Chen, Xin;Tan, Baoyu;Peng, Songlin
通讯作者：
Peng, Songlin
影响因子：
2.9
作者：
Chen, Yang;Dou, Jinhe;Chen, Chuanzhong
通讯作者：
Chen, Chuanzhong