Analysis of the chromosomal clustering of Fusarium-responsive wheat genes uncovers new players in the defence against head blight disease.
Analysis of the chromosomal clustering of Fusarium-responsive wheat genes uncovers new players in the defence against head blight disease.
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DOI：
10.1038/s41598-021-86362-4
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发表时间：
2021-04-02
影响因子：
4.6
通讯作者：
Doohan FM
中科院分区：
文献类型：
--
作者：
Perochon A;Benbow HR;Ślęczka-Brady K;Malla KB;Doohan FM
越来越多的证据表明，一些功能相关的，共表达的基因簇内真核生物基因组。我们提出了一种新的管道，描绘这样的真核基因簇。利用这个工具，我们发现了44个对真菌病原体禾谷镰刀菌（Fusarium graminearum）敏感的基因簇。正如预期的那样，这些镰刀菌响应基因簇（FRGCs）包括代谢基因簇，其中许多与抗病性相关，但迄今为止尚未描述的小麦。然而，大多数FRGCs是非代谢性的，其中许多含有旁系同源物的簇，包括那些涉及植物疾病反应，如谷胱甘肽转移酶，MAP激酶和萌发样蛋白。20个FRGCs编码非同源、非代谢基因（包括防御相关基因）。这些簇之一包括表征的镰刀菌抗性孤儿基因TaFROG.8个FRGCs映射在6个FHB抗性基因座内。染色体7D上的一个小QTL（4.7 Mb）编码8个镰刀菌响应基因，其中5个在FRGC内。这项研究提供了一种新的工具，以确定基因组区域丰富的基因响应感兴趣的特定性状，并在此应用，它突出了基因家族，遗传位点和生物学途径的重要性，在小麦对疾病的反应。
There is increasing evidence that some functionally related, co-expressed genes cluster within eukaryotic genomes. We present a novel pipeline that delineates such eukaryotic gene clusters. Using this tool for bread wheat, we uncovered 44 clusters of genes that are responsive to the fungal pathogen Fusarium graminearum. As expected, these Fusarium-responsive gene clusters (FRGCs) included metabolic gene clusters, many of which are associated with disease resistance, but hitherto not described for wheat. However, the majority of the FRGCs are non-metabolic, many of which contain clusters of paralogues, including those implicated in plant disease responses, such as glutathione transferases, MAP kinases, and germin-like proteins. 20 of the FRGCs encode nonhomologous, non-metabolic genes (including defence-related genes). One of these clusters includes the characterised Fusarium resistance orphan gene, TaFROG. Eight of the FRGCs map within 6 FHB resistance loci. One small QTL on chromosome 7D (4.7 Mb) encodes eight Fusarium-responsive genes, five of which are within a FRGC. This study provides a new tool to identify genomic regions enriched in genes responsive to specific traits of interest and applied herein it highlighted gene families, genetic loci and biological pathways of importance in the response of wheat to disease.
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影响因子：
64.5
作者：
Jeon, Ju Eun;Kim, Jung-Gun;Sattely, Elizabeth
通讯作者：
Sattely, Elizabeth
影响因子：
4.9
作者：
Kettle, Andrew J.;Batley, Jacqueline;Gardiner, Donald M.
通讯作者：
Gardiner, Donald M.
影响因子：
5.3
作者：
Ali SS;Gunupuru LR;Kumar GB;Khan M;Scofield S;Nicholson P;Doohan FM
通讯作者：
Doohan FM
影响因子：
5.4
作者：
Buerstmayr, H;Lemmens, M;Ruckenbauer, P
通讯作者：
Ruckenbauer, P
影响因子：
56.9
作者：
Frey, M;Chomet, P;Gierl, A
通讯作者：
Gierl, A