Composition of elastin like polypeptide-collagen composite scaffold influences in vitro osteogenic activity of human adipose derived stem cells.
Composition of elastin like polypeptide-collagen composite scaffold influences in vitro osteogenic activity of human adipose derived stem cells.
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DOI：
10.1016/j.dental.2016.07.009
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发表时间：
2016-10
期刊：
影响因子：
5
通讯作者：
Janorkar, Amol V.
中科院分区：
文献类型：
--
作者：
Gurumurthy, Bhuuaneswari;Bierdeman, Patrick C.;Janorkar, Amol V.
关键词：
用于引导性骨再生（GBR）的基于胶原的支架不断改进以克服胶原的机械弱点。我们以前已经证明了弹性蛋白样多肽（ELP）增强胶原复合材料的上级机械特性。本研究的目的是评估ELP-胶原复合物培养人脂肪干细胞（hASCs）并使其进行成骨分化的功效。我们假设，与纯胶原水凝胶相比，hASC在更硬的ELP-胶原复合物中将显示出上级成骨分化。通过以3：1的比例改变ELP（0-18 mg/mL）和胶原蛋白（2-6 mg/mL）来制备复合样本。拉伸强度，弹性模量，和韧性测定单轴拉伸试验。在复合材料内培养的hASC通过生物化学测定来表征，以测量细胞活力、蛋白质含量和成骨分化（碱性磷酸酶活性、骨钙素和茜素红染色）。扫描电子显微镜和能量色散谱用于复合材料的形态表征。所有复合物都适合于在22天培养期内与活细胞一起培养hASC。与其他组合物相比，具有18 mg/mL ELP和6 mg/mL胶原的ELP-胶原复合物具有更大的拉伸强度和弹性模量，以及在3周的时间内分化和随后的矿化方面更高的成骨活性。在18：6 mg/mL ELP-胶原复合物中特别观察到由钙和磷组成的细胞外基质沉积物。18：6 mg/mL ELP-胶原复合物成功实现长期三维培养和成骨分化，表明其作为GBR支架的潜力。
Collagen-based scaffolds for guided bone regeneration (GBR) are continuously improved to overcome the mechanical weaknesses of collagen. We have previously demonstrated superior mechanical characteristics of the elastin-like polypeptide (ELP) reinforced collagen composites. The objectives of this research were to evaluate the efficacy of ELP-collagen composites to culture human adipose-derived stem cells (hASCs) and allow them to undergo osteogenic differentiation. We hypothesized that hASCs would show a superior osteogenic differentiation in stiffer ELP-collagen composites compared to the neat collagen hydrogels. Composite specimens were made by varying ELP (0–18 mg/mL) and collagen (2–6 mg/mL) in a 3:1 ratio. Tensile strength, elastic modulus, and toughness were determined by uniaxial tensile testing. hASCs cultured within the composites were characterized by biochemical assays to measure cell viability, protein content, and osteogenic differentiation (alkaline phosphatase activity, osteocalcin, and Alizarin red staining). Scanning electron microscopy and energy dispersive spectroscopy was used for morphological characterization of composites. All composites were suitable for hASCs culture with viable cells over the 22-day culture period. The ELP-collagen composite with 18 mg/mL of ELP and 6 mg/mL of collagen had greater tensile strength and elastic modulus combined with higher osteogenic activity in terms of differentiation and subsequent mineralization over a period of 3 weeks compared to other compositions. The extra-cellular matrix deposits composed of calcium and phosphorous were specifically seen in the 18:6 mg/mL ELP-collagen composite. The success of the 18:6 mg/mL ELP-collagen composite to achieve long-term, 3-dimensional culture and osteogenic differentiation of indicates its potential as a GBR scaffold.
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影响因子：
14
作者：
Banks, Jessica M.;Mozdzen, Laura C.;Harley, Brendan A. C.;Bailey, Ryan C.
通讯作者：
Bailey, Ryan C.
影响因子：
14
作者：
Annabi, Nasim;Mithieux, Suzanne M.;Dehghani, Fariba
通讯作者：
Dehghani, Fariba
影响因子：
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作者：
Heino, J
通讯作者：
Heino, J
影响因子：
3.1
作者：
Lendeckel, S;Jödicke, A;Howaldt, HP
通讯作者：
Howaldt, HP
影响因子：
4.3
作者：
Felipe, Maria Emilia M. C.;Andrade, Patricia F.;Novaes, Arthur B., Jr.
通讯作者：
Novaes, Arthur B., Jr.